V gamma 2V delta 2 TCR-dependent recognition of non-peptide antigens and Daudi cells analyzed by TCR gene transfer

Jack F Bukowski; Craig T Morita; Yoshimasa Tanaka; Barry R Bloom; Michael B Brenner; Hamid Band

doi:10.4049/jimmunol.154.3.998

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V gamma 2V delta 2 TCR-dependent recognition of non-peptide antigens and Daudi cells analyzed by TCR gene transfer

Journal article

Peer reviewed

V gamma 2V delta 2 TCR-dependent recognition of non-peptide antigens and Daudi cells analyzed by TCR gene transfer

Jack F Bukowski, Craig T Morita, Yoshimasa Tanaka, Barry R Bloom, Michael B Brenner and Hamid Band

The Journal of immunology (1950), Vol.154(3), pp.998-1006

02/01/1995

DOI: 10.4049/jimmunol.154.3.998

PMID: 7529807

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Abstract

The predominant subpopulation of gamma delta T cells in human peripheral blood expresses TCR V region genes V gamma 2 paired with V delta 2. Previous studies have shown that these V gamma 2V delta 2+ T cells proliferate in response to Daudi Burkitt lymphoma cells, synthetic alkyl phosphate molecules including monoethylphosphate (MEP), and an Ag chemically similar to MEP purified from mycobacterial extracts of several species including Mycobacterium tuberculosis. This proliferation is polyclonal and determined by the TCR V gene. However, because these alkyl phosphate molecules are so distinct from conventional peptides and superantigens, we questioned whether these substances induce gamma delta T cell proliferation via TCR-dependent recognition. Here we report that transfection of TCR- Jurkat T cells with cDNA constructs encoding a V gamma 2V delta 2 TCR enabled the transfectants to produce IL-2 in response to Daudi cells, mycobacterial extract, and MEP. The responses were dose dependent and Ag specific. These results demonstrate an essential role for the gamma delta TCR in V gamma 2V delta 2 T cell-mediated recognition of non-peptide Ags by human T cells and suggest a structural similarity or cross-reactivity between cellular and microbial Ags recognized by these gamma delta T cells.

Epitopes - immunology

Tumor Cells, Cultured - immunology

Burkitt Lymphoma - immunology

Mycobacterium - immunology

Transfection - genetics

Base Sequence

Humans

Antigens, Bacterial - immunology

Organophosphates - immunology

Molecular Sequence Data

Receptors, Antigen, T-Cell, gamma-delta - genetics

Interleukin-2 - secretion

Details

Title: Subtitle: V gamma 2V delta 2 TCR-dependent recognition of non-peptide antigens and Daudi cells analyzed by TCR gene transfer
Creators: Jack F Bukowski
Craig T Morita
Yoshimasa Tanaka
Barry R Bloom
Michael B Brenner
Hamid Band - Brigham and Women's Hospital
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.154(3), pp.998-1006
DOI: 10.4049/jimmunol.154.3.998
PMID: 7529807
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: AI28508 / NIAID NIH HHS CA47724 / NCI NIH HHS AR36308 / NIAMS NIH HHS
Language: English
Date published: 02/01/1995
Academic Unit: Immunology; Internal Medicine
Record Identifier: 9984094607802771

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