Journal article
Vaccination against Staphylococcus aureus pneumonia
The Journal of infectious diseases, Vol.209(12), pp.1955-1962
06/15/2014
DOI: 10.1093/infdis/jit823
PMCID: PMC4038136
PMID: 24357631
Abstract
Staphylococcus aureus causes serious infections in both hospital and community settings. Attempts have been made to prevent human infection through vaccination against bacterial cell-surface antigens; thus far all have failed. Here we show that superantigens and cytolysins, when used in vaccine cocktails, provide protection from S. aureus USA100-USA400 intrapulmonary challenge.
Rabbits were actively vaccinated (wild-type toxins or toxoids) or passively immunized (hyperimmune serum) against combinations of superantigens (toxic shock syndrome toxin 1, enterotoxins B and C, and enterotoxin-like X) and cytolysins (α-, β-, and γ-toxins) and challenged intrapulmonarily with multiple strains of S. aureus, both methicillin-sensitive and methicillin-resistant.
Active vaccination against a cocktail containing bacterial cell-surface antigens enhanced disease severity as tested by infective endocarditis. Active vaccination against secreted superantigens and cytolysins resulted in protection of 86 of 88 rabbits when challenged intrapulmonarily with 9 different S. aureus strains, compared to only 1 of 88 nonvaccinated animals. Passive immunization studies demonstrated that production of neutralizing antibodies was an important mechanism of protection.
The data suggest that vaccination against bacterial cell-surface antigens increases disease severity, but vaccination against secreted virulence factors provides protection against S. aureus. These results advance our understanding of S. aureus pathogenesis and have important implications in disease prevention.
Details
- Title: Subtitle
- Vaccination against Staphylococcus aureus pneumonia
- Creators
- Adam R Spaulding - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa CityWilmara Salgado-Pabón - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa CityJoseph A Merriman - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa CityChristopher S Stach - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa CityYinduo Ji - Department of Veterinary Biosciences, College of Veterinary MedicineAaron N Gillman - Department of Experimental and Clinical Pharmacology, College of Pharmacy, University of Minnesota, MinneapolisMarnie L Peterson - Department of Experimental and Clinical Pharmacology, College of Pharmacy, University of Minnesota, MinneapolisPatrick M Schlievert - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.209(12), pp.1955-1962
- Publisher
- United States
- DOI
- 10.1093/infdis/jit823
- PMID
- 24357631
- PMCID
- PMC4038136
- ISSN
- 1537-6613
- eISSN
- 1537-6613
- Grant note
- R01 AI074283 / NIAID NIH HHS U54 AI057153 / NIAID NIH HHS R01 AI073366 / NIAID NIH HHS AI73366 / NIAID NIH HHS AI074283 / NIAID NIH HHS AI57153 / NIAID NIH HHS
- Language
- English
- Date published
- 06/15/2014
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001209202771
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