Vaccinia Virus Entry, Exit, and Interaction with Differentiated Human Airway Epithelia

Paola D Vermeer; Julia McHugh; Tatiana Rokhlina; Daniel W Vermeer; Joseph Zabner; Michael J Welsh

doi:10.1128/JVI.00601-07

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Vaccinia Virus Entry, Exit, and Interaction with Differentiated Human Airway Epithelia

Journal article

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Vaccinia Virus Entry, Exit, and Interaction with Differentiated Human Airway Epithelia

Paola D Vermeer, Julia McHugh, Tatiana Rokhlina, Daniel W Vermeer, Joseph Zabner and Michael J Welsh

Journal of virology, Vol.81(18), pp.9891-9899

09/2007

DOI: 10.1128/JVI.00601-07

PMID: 17581984

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Abstract

Variola virus, the causative agent of smallpox, enters and exits the host via the respiratory route. To better understand the pathogenesis of poxvirus infection and its interaction with respiratory epithelia, we used vaccinia virus and examined its interaction with primary cultures of well-differentiated human airway epithelia. We found that vaccinia virus preferentially infected the epithelia through the basolateral membrane and released viral progeny across the apical membrane. Despite infection and virus production, epithelia retained tight junctions, transepithelial electrical conductance, and a steep transepithelial concentration gradient of virus, indicating integrity of the epithelial barrier. In fact, during the first four days of infection, epithelial height and cell number increased. These morphological changes and maintenance of epithelial integrity required vaccinia virus growth factor, which was released basolaterally, where it activated epidermal growth factor 1 receptors. These data suggest a complex interaction between the virus and differentiated airway epithelia; the virus preferentially enters the cells basolaterally, exits apically, and maintains epithelial integrity by stimulating growth factor receptors.

Pathogenesis and Immunity

Details

Title: Subtitle: Vaccinia Virus Entry, Exit, and Interaction with Differentiated Human Airway Epithelia
Creators: Paola D Vermeer - Departments of Internal Medicine
Julia McHugh - Departments of Internal Medicine
Tatiana Rokhlina - Departments of Internal Medicine
Daniel W Vermeer - Departments of Internal Medicine
Joseph Zabner - Departments of Internal Medicine
Michael J Welsh - Departments of Internal Medicine
Resource Type: Journal article
Publication Details: Journal of virology, Vol.81(18), pp.9891-9899
DOI: 10.1128/JVI.00601-07
PMID: 17581984
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: American Society for Microbiology
Language: English
Date published: 09/2007
Academic Unit: Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Neurosurgery; Internal Medicine
Record Identifier: 9984020651302771

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