Validation of 24-Hour Fluconazole MIC Readings versus the CLSI 48-Hour Broth Microdilution Reference Method: Results from a Global Candida Antifungal Surveillance Program

M. A Pfaller; L. B Boyken; R. J Hollis; J Kroeger; S. A Messer; S Tendolkar; D. J Diekema

doi:10.1128/JCM.01391-08

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Validation of 24-Hour Fluconazole MIC Readings versus the CLSI 48-Hour Broth Microdilution Reference Method: Results from a Global Candida Antifungal Surveillance Program

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Validation of 24-Hour Fluconazole MIC Readings versus the CLSI 48-Hour Broth Microdilution Reference Method: Results from a Global Candida Antifungal Surveillance Program

M. A Pfaller, L. B Boyken, R. J Hollis, J Kroeger, S. A Messer, S Tendolkar and D. J Diekema

Journal of clinical microbiology, Vol.46(11), pp.3585-3590

11/2008

DOI: 10.1128/JCM.01391-08

PMCID: PMC2576604

PMID: 18784314

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Abstract

We performed 24- and 48-h MIC determinations and disk diffusion testing of fluconazole against more than 11,000 clinical isolates of Candida species. By using the reference MIC breakpoints, the categorical agreement between the 24-h and reference 48-h broth microdilution results ranged from 93.8% (all Candida species) to 94.9% (all Candida species minus Candida krusei ), with only 0.1% very major errors (VME). The essential agreement (within 2 log 2 dilutions) between the 24-h and 48-h results was 99.6%. The categorical agreement between the 24-h disk diffusion results and the 24-h MIC results, using the previously established breakpoints, was 94.4%, with 0.1% VME. Both the MIC and the disk diffusion results obtained for fluconazole after only 24 h of incubation may be used to determine the susceptibilities of Candida spp. to this widely used antifungal agent.

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Title: Subtitle: Validation of 24-Hour Fluconazole MIC Readings versus the CLSI 48-Hour Broth Microdilution Reference Method: Results from a Global Candida Antifungal Surveillance Program
Creators: M. A Pfaller - Departments of Pathology
L. B Boyken - Departments of Pathology
R. J Hollis - Departments of Pathology
J Kroeger - Departments of Pathology
S. A Messer - Departments of Pathology
S Tendolkar - Departments of Pathology
D. J Diekema - Roy J. and Lucille A. Carver College of Medicine
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.46(11), pp.3585-3590
DOI: 10.1128/JCM.01391-08
PMID: 18784314
PMCID: PMC2576604
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: American Society for Microbiology (ASM)
Language: English
Date published: 11/2008
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9984185277602771

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