Validation of 24-hour posaconazole and voriconazole MIC readings versus the CLSI 48-hour broth microdilution reference method: application of epidemiological cutoff values to results from a global Candida antifungal surveillance program

M A Pfaller; L B Boyken; R J Hollis; J Kroeger; S A Messer; S Tendolkar; D J Diekema

doi:10.1128/JCM.02437-10

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Validation of 24-hour posaconazole and voriconazole MIC readings versus the CLSI 48-hour broth microdilution reference method: application of epidemiological cutoff values to results from a global Candida antifungal surveillance program

Journal article

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Validation of 24-hour posaconazole and voriconazole MIC readings versus the CLSI 48-hour broth microdilution reference method: application of epidemiological cutoff values to results from a global Candida antifungal surveillance program

M A Pfaller, L B Boyken, R J Hollis, J Kroeger, S A Messer, S Tendolkar and D J Diekema

Journal of clinical microbiology, Vol.49(4), pp.1274-1279

04/2011

DOI: 10.1128/JCM.02437-10

PMCID: PMC3122818

PMID: 21289155

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Abstract

We performed 24- and 48-h MIC determinations of posaconazole and voriconazole against more than 16,000 clinical isolates of Candida species. By using the 24- and 48-h epidemiological cutoff values (ECVs), the categorical agreement between the 24-h and reference 48-h broth microdilution results ranged from 97.1% (C. parapsilosis and voriconazole) to 99.8% (C. krusei and voriconazole), with 0.0 to 2.9% very major discrepancies (VMD). The essential agreement (within 2 log(2) dilutions) between the 24- and 48-h results was 99.6% for both posaconazole and voriconazole. The MIC results obtained for both posaconazole and voriconazole after only 24 h of incubation may be used to determine the susceptibilities of Candida spp. to these important antifungal agents. The applications of ECVs to this large collection of Candida isolates suggests the potential to develop 24-h species-specific clinical breakpoints for both posaconazole and voriconazole.

Antifungal Agents - pharmacology

Triazoles - pharmacology

Time Factors

Humans

Voriconazole

Candida - drug effects

Pyrimidines - pharmacology

Microbial Sensitivity Tests - methods

Details

Title: Subtitle: Validation of 24-hour posaconazole and voriconazole MIC readings versus the CLSI 48-hour broth microdilution reference method: application of epidemiological cutoff values to results from a global Candida antifungal surveillance program
Creators: M A Pfaller - Medical Microbiology Division, C606 GH, Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242, USA. michael-pfaller@uiowa.edu
L B Boyken
R J Hollis
J Kroeger
S A Messer
S Tendolkar
D J Diekema
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.49(4), pp.1274-1279
DOI: 10.1128/JCM.02437-10
PMID: 21289155
PMCID: PMC3122818
NLM abbreviation: J Clin Microbiol
ISSN: 1098-660X
eISSN: 1098-660X
Publisher: United States
Language: English
Date published: 04/2011
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986367002771

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