Journal article
Validation of tissue microarrays using p53 immunohistochemical studies of squamous cell carcinoma of the larynx
Modern pathology, Vol.16(12), pp.1181-1188
12/2003
DOI: 10.1097/01.MP.0000097284.40421.D6
PMID: 14681317
Abstract
Tissue microarrays are a powerful new tissue-conserving technology in the study of cancer, allowing simultaneous study of a large number of tumor specimens. We sought to ascertain the utility of tissue microarrays in head and neck cancer pathology using squamous cell carcinoma of the larynx as a model system. Whole-specimen slides from 44 different laryngeal squamous cell carcinomas were stained for p53 expression. Microarrays were then generated by taking six 0.6-mm core biopsies from each of the 44 specimens. The whole sections and the microarrays were independently scored for p53 expression. Twenty-three (53%) of the 44 tumor specimens were positive for p53. Forty-four of the 264 core biopsies (17%) were not given a score because of the lack of tumor cells. Seventy-eight percent of the individual discs on the microarray had scores in agreement with those of the whole-section slides. Among biopsy discs with tumor cells present, 94.5% were in agreement with the whole-section slide. The average probability that four randomly chosen biopsy discs, considered together, would accurately identify the presence of p53 staining in a whole section was 0.97 (95% CI.93-1.0). We conclude that tissue microarrays for squamous cell carcinomas can accurately represent immunohistochemical results of whole-slide specimens when four or more samples are used. Tissue microarrays are an important technique that may be applied to immunohistochemical studies of head and neck cancer.
Details
- Title: Subtitle
- Validation of tissue microarrays using p53 immunohistochemical studies of squamous cell carcinoma of the larynx
- Creators
- Mark C Griffin - Department of Otolaryngology/Head and Neck Surgery, University of Iowa Health Care, Iowa City, Iowa 52242, USARobert A RobinsonDouglas K Trask
- Resource Type
- Journal article
- Publication Details
- Modern pathology, Vol.16(12), pp.1181-1188
- Publisher
- United States
- DOI
- 10.1097/01.MP.0000097284.40421.D6
- PMID
- 14681317
- ISSN
- 0893-3952
- eISSN
- 1530-0285
- Language
- English
- Date published
- 12/2003
- Academic Unit
- Oral Pathology, Radiology and Medicine; Pathology
- Record Identifier
- 9984047897002771
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