Journal article
Vanillin-bioglass cross-linked 3D porous chitosan scaffolds with strong osteopromotive and antibacterial abilities for bone tissue engineering
Carbohydrate polymers, Vol.271, pp.118440-118440
11/01/2021
DOI: 10.1016/j.carbpol.2021.118440
PMCID: PMC8353169
PMID: 34364578
Abstract
Chitosan scaffolds crosslinked by current methods insufficiently meet the demands of bone tissue engineering applications. We developed a novel effective crosslinking technique by using the natural and safe vanillin together with bioglass microparticles to generate an antibacterial, osteoconductive, and mechanically robust 3D porous chitosan-vanillin-bioglass (CVB) scaffold. In addition to the significantly improved mechanical properties, the CVB scaffolds had high porosity (>90%) and interconnected macroporous structures. Our data suggested that the crosslinking mainly resulted from the Schiff base reactions between the aldehydes of vanillin and amines of chitosan, together with the hydrogen and ionic bonds formed within them. Importantly, the CVB scaffolds not only showed good biocompatibility, bioactivity, and strong antibacterial ability but also significantly promoted osteoblastic differentiation, mineralization in vitro, and ectopic bone formation in vivo. Thus, the CVB scaffolds hold great promise for bone tissue engineering applications based on their robust mechanical properties, osteoconductivity, and antibacterial abilities.
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•A novel vanillin-bioglass microparticles-based crosslinking method for chitosan scaffold•Innovative 3D porous chitosan-vanillin-BG (CVB) scaffold was developed.•CVB showed significantly improved mechanical properties, stability, and bioactivity.•MC3T3-E1 cells showed significantly enhanced osteoblastic differentiation on CVB.•CVB showed strong antibacterial activity and osteoconductivity in vivo.
Details
- Title: Subtitle
- Vanillin-bioglass cross-linked 3D porous chitosan scaffolds with strong osteopromotive and antibacterial abilities for bone tissue engineering
- Creators
- Jue Hu - Department of Oral and Maxillofacial Surgery, University of Iowa College of Dentistry, Iowa City, IA 52242, USAZhuozhi Wang - Department of Oral and Maxillofacial Surgery, University of Iowa College of Dentistry, Iowa City, IA 52242, USAJacob M Miszuk - Department of Oral and Maxillofacial Surgery, University of Iowa College of Dentistry, Iowa City, IA 52242, USAMin Zhu - Iowa Institute for Oral Health Research, University of Iowa College of Dentistry, Iowa City, IA 52242, USAThiranjeewa I Lansakara - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USAAlexei V Tivanski - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USAJeffrey A Banas - Iowa Institute for Oral Health Research, University of Iowa College of Dentistry, Iowa City, IA 52242, USAHongli Sun - Department of Oral and Maxillofacial Surgery, University of Iowa College of Dentistry, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Carbohydrate polymers, Vol.271, pp.118440-118440
- DOI
- 10.1016/j.carbpol.2021.118440
- PMID
- 34364578
- PMCID
- PMC8353169
- NLM abbreviation
- Carbohydr Polym
- ISSN
- 0144-8617
- eISSN
- 1879-1344
- Publisher
- Elsevier Ltd
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: R01DE029159, T90DE023520; DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research
- Language
- English
- Date published
- 11/01/2021
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pediatric Dentistry; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Chemistry; Dental Research
- Record Identifier
- 9984216580302771
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