Journal article
Variability in the carbazole assay for N-desulfonated/N-acylated heparin derivatives
Carbohydrate research, Vol.342(17), pp.2664-2669
12/10/2007
DOI: 10.1016/j.carres.2007.08.004
PMID: 17850773
Abstract
The carbazole assay is commonly employed to quantify heparin and other uronic acid-containing polysaccharides. Heparin-derived standard curves are often employed to quantify solutions of various natural and unnatural heparin structures that have different levels of sulfate substitution, different levels of N-sulfo and N-acetyl groups, and other structural changes as a consequence of reducing molecular weight. Recent studies in our laboratory have focused on chemically modified heparin derivatives comprised of structurally diverse N-acyl moieties substituted into heparin in place of N-sulfo groups. We report here that although differing degrees of 2-N-sulfo-, 2-N-acetyl- or 2-amino-D-glucosamine residues within heparin do not affect signal intensity in the carbazole assay, replacing N-sulfo groups in heparin with structurally diverse N-acyl moieties affords products that display significant variation in the assay. The structure of different N-acyl groups, and to a lesser extent the degree of N-acylation by individual N-acyl groups, is shown to variably alter signal intensity in the carbazole assay even though content and structure of uronic acid residues is unaltered. (c) 2007 Elsevier Ltd. All rights reserved.
Details
- Title: Subtitle
- Variability in the carbazole assay for N-desulfonated/N-acylated heparin derivatives
- Creators
- Christopher M. Hattan - University of IowaRobert J. Kerns - Univ Iowa, Div Med & Nat Prod Chem, Iowa City, IA 52242 USA
- Resource Type
- Journal article
- Publication Details
- Carbohydrate research, Vol.342(17), pp.2664-2669
- Publisher
- Elsevier
- DOI
- 10.1016/j.carres.2007.08.004
- PMID
- 17850773
- ISSN
- 0008-6215
- eISSN
- 1873-426X
- Number of pages
- 6
- Language
- English
- Date published
- 12/10/2007
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984365901202771
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