Journal article
Variable Effects of Autophagy Induction by Trehalose on Herpesviruses Depending on Conditions of Infection
The Yale journal of biology & medicine, Vol.90(1), pp.25-33
03/01/2017
PMCID: PMC5369042
PMID: 28356891
Abstract
Trehalose is a non-reducing sugar formed from two glucose units. Trehalose induces abundant autophagy in cultured cells and also reduces the rate of aggregation of the huntingtin protein in the animal model of Huntington disease, a chronic neurological disease in humans. The mechanism of this effect on autophagy is now known to be caused by starvation secondary to inhibition of a family of glucose transporters known as the solute carrier 2 or the glucose transporter family. Variable effects of trehalose treatment have been observed during infections with two herpesviruses-human cytomegalov irus and varicella-zoster virus. The reasons for differing results have now been delineated. These differences are caused by two variables in conditions of infection: timing of addition of trehalose and type of inoculum (cell-free virus vs. infected cells). When monolayers pretreated with trehalose were inoculated with cell-free virus, there was a decline in virus spread by as much as 93 percent when compared with untreated monolayers. However, when monolayers were inoculated with infected cells rather than cell-free virus, there was no decline in virus spread. These results demonstrated that the effect of trehalose was limited to monolayers that were starved when inoculated with cell-free virus. In contrast, sufficient virus was already present in infected cell inocula so as to minimize any inhibitory effect of a starved monolayer. These results also showed that trehalose did not specifically inhibit a herpesvirus; rather, addition of trehalose to cell culture media altered the intracellular environment.
Details
- Title: Subtitle
- Variable Effects of Autophagy Induction by Trehalose on Herpesviruses Depending on Conditions of Infection
- Creators
- Jeffery L. Meier - Univ Iowa, Dept Internal Med, Virol Labs, Iowa City, IA 52242 USACharles Grose - Univ Iowa, Dept Psychiat, Iowa City, IA 52242 USA
- Resource Type
- Journal article
- Publication Details
- The Yale journal of biology & medicine, Vol.90(1), pp.25-33
- Publisher
- Yale J Biology Medicine, Inc
- PMID
- 28356891
- PMCID
- PMC5369042
- ISSN
- 0044-0086
- eISSN
- 1551-4056
- Number of pages
- 9
- Grant note
- AI89716 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 03/01/2017
- Academic Unit
- Infectious Diseases; Stead Family Department of Pediatrics; Epidemiology; Infectious Disease (Pediatrics); Internal Medicine
- Record Identifier
- 9984353846002771
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