Journal article
Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region
Science (American Association for the Advancement of Science), Vol.309(5731), pp.142-145
2005
DOI: 10.1126/science.1111915
PMID: 15994560
Abstract
Cell signaling that culminates in posttranslational modifications directs protein activity. Here we report how multiple $Ca^{2+}-dependent$ phosphorylation sites within the transcription activator Ets-1 act additively to produce graded DNA binding affinity. Nuclear magnetic resonance spectroscopic analyses show that phosphorylation shifts Ets-1 from a dynamic conformation poised to bind DNA to a well-folded inhibited state. These phosphates lie in an unstructured flexible region that functions as the allosteric effector of autoinhibition. Variable phosphorylation thus serves as a "rheostat" for cell signaling to fine-tune transcription at the level of DNA binding.
Details
- Title: Subtitle
- Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region
- Creators
- Miles A PUFALL - Huntsman Cancer Institute, Department of Oncological Sciences, University of Utah, Salt Lake City, UT 84112-5550, United StatesGregory M LEE - Department of Biochemistry and Molecular Biology, Department of Chemistry, and The Michael Smith Laboratory, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, CanadaMary L NELSON - Huntsman Cancer Institute, Department of Oncological Sciences, University of Utah, Salt Lake City, UT 84112-5550, United StatesHyun-Seo KANG - Department of Biochemistry and Molecular Biology, Department of Chemistry, and The Michael Smith Laboratory, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, CanadaAlgirdas VELYVIS - Departments of Medical Genetics, Biochemistry, and Chemistry, University of Toronto, Toronto, Ontario, M5S 1A8, CanadaLewis E KAY - Departments of Medical Genetics, Biochemistry, and Chemistry, University of Toronto, Toronto, Ontario, M5S 1A8, CanadaLawrence P MCINTOSH - Department of Biochemistry and Molecular Biology, Department of Chemistry, and The Michael Smith Laboratory, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, CanadaBarbara J GRAVES - Huntsman Cancer Institute, Department of Oncological Sciences, University of Utah, Salt Lake City, UT 84112-5550, United States
- Resource Type
- Journal article
- Publication Details
- Science (American Association for the Advancement of Science), Vol.309(5731), pp.142-145
- DOI
- 10.1126/science.1111915
- PMID
- 15994560
- NLM abbreviation
- Science
- ISSN
- 0036-8075
- eISSN
- 1095-9203
- Publisher
- American Association for the Advancement of Science; Washington, DC
- Language
- English
- Date published
- 2005
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984024526402771
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