Journal article
Variation in nuclear genome size in a freshwater snail model system featuring a recent whole-genome duplication
Royal Society open science, Vol.12(6), 250171
06/2025
DOI: 10.1098/rsos.250171
PMCID: PMC12133333
PMID: 40469660
Appears in UI Libraries Support Open Access
Abstract
Conspecifics often share—or are assumed to share—nuclear genome characteristics like nucleotide composition and genome size. These fundamental aspects of the nuclear genome can themselves be the object of natural selection. We here provide the first high-quality direct measurements of nuclear genome DNA content in a representative diverse sample of Potamopyrgus antipodarum, an Aotearoa New Zealand freshwater snail that is a textbook example of the maintenance of sexual reproduction in nature and is invasive worldwide. We used propidium-iodide-based flow cytometry to characterize nuclear DNA content and its variation in nearly 100 P. antipodarum from multiple populations representing both sexual and asexual individuals. We also estimated nuclear DNA content in multiple P. estuarinus, a closely related obligately sexual species. These data confirmed and extended earlier lines of evidence for polyploidy and variable genome size within asexual P. antipodarum and provided the first direct demonstration of distinctly higher nuclear genome content in diploid (sexual) P. antipodarum relative to diploid sexual P. estuarinus. Together, these results are consistent with genomic evidence for a recent whole-genome duplication (WGD) and subsequent and in-process rediploidization in P. antipodarum, setting the stage for use of Potamopyrgus as a model for WGD and its consequences.
Details
- Title: Subtitle
- Variation in nuclear genome size in a freshwater snail model system featuring a recent whole-genome duplication
- Creators
- Maurine Neiman - University of Iowa, BiologyMaria PichlerMartin HaaseDunja K Lamatsch
- Resource Type
- Journal article
- Publication Details
- Royal Society open science, Vol.12(6), 250171
- DOI
- 10.1098/rsos.250171
- PMID
- 40469660
- PMCID
- PMC12133333
- NLM abbreviation
- R Soc Open Sci
- ISSN
- 2054-5703
- Publisher
- Royal Society
- Grant note
- NSF: MCB-1122176, DEB-1753851 University of Iowa International ProgramsGerman Science Foundation
Funding was provided by NSF MCB-1122176 and NSF DEB-1753851. Professor Manfred Schartl also contributed funds to support flow cytometry analyses. We thank the University of Iowa International Programs for International Travel Awards that supported travel for data collection and analysis in 2019 and 2021, and the University of Innsbruck for a guest professorship to Maurine Neiman in 2019 and 2025. Some of the collections were made in the frame of the Research Training Group 2010 RESPONSE funded by the German Science Foundation.
- Language
- English
- Date published
- 06/2025
- Academic Unit
- Gender, Women's and Sexuality Studies; Biology
- Record Identifier
- 9984821748602771
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