Journal article
Variation in recombinant protein expression levels among clones of lepidopteran cell populations
Enzyme and microbial technology, Vol.17(2), pp.168-174
1995
DOI: 10.1016/0141-0229(94)00018-M
Abstract
Clones isolated from the heterogenous
Spodoptera frugiperda IPLB-Sf-21-AE and
Mamestra brassiace IZD-Mb-O503 insect cell populations have been compared in regards to their synthesis of recombinant
Escherichia coli β-galactosidase (β-gal), human tissue plasminogen activator (t-PA), and human kidney renin. Significant differences were found among the clones in their expression levels of these proteins. In addition, the expression levels oft-PA and renin were considerably less than that of β-gal. Clone BC5 was the best producer of t-PA and renin among the Mb-O503 clones, whereas clone BB5 was the best producer of β-gal. The Sf-9 cell line and clone 2 were the best producers of t-PA and renin, respectively, among the Sf-21 clones. The Mb-O503 clones generally were better producers of t-PA than the Sf-21 clones, with the BC5 clone producing twice the levels of any other Mb-O503 or Sf-21 clone. The Sf-21 clones were generally better renin producers; the Mb-O503 clones were generally found to have β-gal expression levels superior to those reported previously for the Sf-21 clones.
Details
- Title: Subtitle
- Variation in recombinant protein expression levels among clones of lepidopteran cell populations
- Creators
- Murali K PasumarthyDavid W Murhammer
- Resource Type
- Journal article
- Publication Details
- Enzyme and microbial technology, Vol.17(2), pp.168-174
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0141-0229(94)00018-M
- ISSN
- 0141-0229
- eISSN
- 1879-0909
- Language
- English
- Date published
- 1995
- Academic Unit
- Chemical and Biochemical Engineering
- Record Identifier
- 9984003468702771
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