Journal article
Variation of mechanical properties and quantitative proteomics of VSMC along the arterial tree
American journal of physiology. Heart and circulatory physiology, Vol.306(4), pp.H505-H516
02/15/2014
DOI: 10.1152/ajpheart.00655.2013
PMID: 24337458
Abstract
Vascular smooth muscle cells (VSMCs) are thought to assume a quiescent and homogeneous mechanical behavior after arterial tree development phase. However, VSMCs are known to be molecularly heterogeneous in other aspects and their mechanics may play a role in pathological situations. Our aim was to evaluate VSMCs from different arterial beds in terms of mechanics and proteomics, as well as investigate factors that may influence this phenotype. VSMCs obtained from seven arteries were studied using optical magnetic twisting cytometry (both in static state and after stretching) and shotgun proteomics. VSMC mechanical data were correlated with anatomical parameters and ultrastructural images of their vessels of origin. Femoral, renal, abdominal aorta, carotid, mammary, and thoracic aorta exhibited descending order of stiffness (G, P < 0.001). VSMC mechanical data correlated with the vessel percentage of elastin and amount of surrounding extracellular matrix (ECM), which decreased with the distance from the heart. After 48 h of stretching simulating regional blood flow of elastic arteries, VSMCs exhibited a reduction in basal rigidity. VSMCs from the thoracic aorta expressed a significantly higher amount of proteins related to cytoskeleton structure and organization vs. VSMCs from the femoral artery. VSMCs are heterogeneous in terms of mechanical properties and expression/organization of cytoskeleton proteins along the arterial tree. The mechanical phenotype correlates with the composition of ECM and can be modulated by cyclic stretching imposed on VSMCs by blood flow circumferential stress.
Details
- Title: Subtitle
- Variation of mechanical properties and quantitative proteomics of VSMC along the arterial tree
- Creators
- Carla Luana Dinardo - Universidade de São PauloGabriela Venturini - Universidade de São PauloEnhua H. Zhou - Harvard UniversityIi Sei Watanabe - Universidade de São PauloLuciene Cristina Gastalho Campos - Universidade de São PauloRafael Dariolli - Universidade de São PauloJoaquim Mauricio da Motta-Leal-Filho - Universidade de São PauloValdemir Melechco Carvalho - Fleury S.A. (Brazil)Karina Helena Morais Cardozo - Fleury Grp, Sao Paulo, BrazilJose Eduardo Krieger - Universidade de São PauloAdriano Mesquita Alencar - Universidade de São PauloAlexandre Costa Pereira - Universidade de São Paulo
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.306(4), pp.H505-H516
- DOI
- 10.1152/ajpheart.00655.2013
- PMID
- 24337458
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Publisher
- Amer Physiological Soc
- Number of pages
- 12
- Grant note
- 12/21103-0 / Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Fleury Group, Sao Paulo, Brazil Sao Paulo Research Foundation (FAPESP); Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
- Language
- English
- Date published
- 02/15/2014
- Academic Unit
- Radiology
- Record Identifier
- 9985180927802771
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