Journal article
Variations at a quantitative trait locus (QTL) affect development of behavior in lead-exposed Drosophila melanogaster
Neurotoxicology (Park Forest South), Vol.30(2), pp.305-311
03/01/2009
DOI: 10.1016/j.neuro.2009.01.004
PMCID: PMC3734858
PMID: 19428504
Abstract
We developed Drosophila melanogaster as a model to study correlated behavioral, neuronal and genetic effects of the neurotoxin lead, known to affect cognitive and behavioral development in children. We showed that, as in vertebrates, lead affects both synaptic development and complex behaviors (courtship, fecundity, locomotor activity) in Drosophila. By assessing differential behavioral responses to developmental lead exposure among recombinant inbred Drosophila lines (RI), derived from parental lines Oregon R and Russian 2b, we have now identified a genotype by environment interaction (GEI) for a behavioral trait affected by lead. Drosophila Activity Monitors (TriKinetics, Waltham, MA), which measure activity by counting the number of times a single fly in a small glass tube walk!; through an infrared beam aimed at the middle of the tube, were used to measure activity of flies, reared from eggs to 4 days of adult age on either control or lead-contaminated medium, from each of 75 RI lines. We observed a significant statistical association between the effect of lead on Average Daytime Activity (ADA) across lines and one marker locus, 30AB, on chromosome 2: we define this as a Quantitative Trait Locus (QTL) associated with behavioral effects of developmental lead exposure. When 30AB was from Russian 2b, lead significantly increased locomotor activity, whereas, when 30AB was from Oregon R, lead decreased it. 30AB contains about 125 genes among which are likely "candidate genes" for the observed lead-dependent behavioral changes. Drosophila are thus a useful, underutilized model for studying behavioral, synaptic and genetic changes following chronic exposure to lead or other neurotoxins during development. (C) 2009 Elsevier Inc. All rights reserved.
Details
- Title: Subtitle
- Variations at a quantitative trait locus (QTL) affect development of behavior in lead-exposed Drosophila melanogaster
- Creators
- Helmut V. B. Hirsch - University at Albany, State University of New YorkDebra Possidente - Skidmore CollegeSarah Averill - University at Albany, State University of New YorkTamira Palmetto Despain - University at Albany, State University of New YorkJoel Buytkins - University at Albany, State University of New YorkValerie Thomas - University at Albany, State University of New YorkW. Paul Goebel - University at Albany, State University of New YorkAsante Shipp-Hilts - University at Albany, State University of New YorkDiane Wilson - University at Albany, State University of New YorkKurt Hollocher - Union CollegeBernard Possidente - Skidmore CollegeGreg Lnenicka - University at Albany, State University of New YorkDouglas M. Ruden - Wayne State University
- Resource Type
- Journal article
- Publication Details
- Neurotoxicology (Park Forest South), Vol.30(2), pp.305-311
- DOI
- 10.1016/j.neuro.2009.01.004
- PMID
- 19428504
- PMCID
- PMC3734858
- NLM abbreviation
- Neurotoxicology
- ISSN
- 0161-813X
- eISSN
- 1872-9711
- Publisher
- Elsevier
- Number of pages
- 7
- Grant note
- P30ES006639 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS) P30 ES06639 / Environmental Health Sciences Center in Molecular and Cellular Toxicology with Human Applications R01 ES01 2933; R01 CA105349 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01CA105349 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Language
- English
- Date published
- 03/01/2009
- Academic Unit
- Radiology
- Record Identifier
- 9984318709102771
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