Varicella-Zoster Virus ORF47 Protein Serine Kinase: Characterization of a Cloned, Biologically Active Phosphotransferase and Two Viral Substrates, ORF62 and ORF63

T. K Kenyon; J Lynch; J Hay; W Ruyechan; C Grose

doi:10.1128/JVI.75.18.8854-8858.2001

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Varicella-Zoster Virus ORF47 Protein Serine Kinase: Characterization of a Cloned, Biologically Active Phosphotransferase and Two Viral Substrates, ORF62 and ORF63

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Varicella-Zoster Virus ORF47 Protein Serine Kinase: Characterization of a Cloned, Biologically Active Phosphotransferase and Two Viral Substrates, ORF62 and ORF63

T. K Kenyon, J Lynch, J Hay, W Ruyechan and C Grose

Journal of virology, Vol.75(18), pp.8854-8858

09/15/2001

DOI: 10.1128/JVI.75.18.8854-8858.2001

PMCID: PMC115131

PMID: 11507231

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Abstract

ABSTRACT Varicella-zoster virus (VZV) codes for a protein serine kinase called ORF47; the herpes simplex virus (HSV) homolog is UL13. No recombinant alphaherpesvirus serine kinase has been biologically active in vitro. We discovered that preservation of the intrinsic kinase activity of recombinant VZV ORF47 required unusually stringent in vitro conditions, including physiological concentrations of polyamines. In this assay, ORF47 phosphorylated two VZV regulatory proteins: the ORF62 protein (homolog of HSV ICP4) and the ORF63 protein (homolog of HSV ICP22). Of interest, ORF47 kinase also coprecipitated ORF63 protein from the kinase assay supernatant.

Details

Title: Subtitle: Varicella-Zoster Virus ORF47 Protein Serine Kinase: Characterization of a Cloned, Biologically Active Phosphotransferase and Two Viral Substrates, ORF62 and ORF63
Creators: T. K Kenyon - Departments of Microbiology and Pediatrics, University of Iowa, Iowa City, Iowa,1 and
J Lynch - Department of Microbiology, State University of New York, Buffalo, New York2
J Hay - Department of Microbiology, State University of New York, Buffalo, New York2
W Ruyechan - Department of Microbiology, State University of New York, Buffalo, New York2
C Grose - Departments of Microbiology and Pediatrics, University of Iowa, Iowa City, Iowa,1 and
Resource Type: Journal article
Publication Details: Journal of virology, Vol.75(18), pp.8854-8858
DOI: 10.1128/JVI.75.18.8854-8858.2001
PMID: 11507231
PMCID: PMC115131
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Language: English
Date published: 09/15/2001
Academic Unit: Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
Record Identifier: 9984093598102771

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