Journal article
Varicella-zoster viral glycoprotein envelopment: ultrastructural cytochemical localization
The journal of histochemistry and cytochemistry, Vol.34(2), pp.281-284
1986
DOI: 10.1177/34.2.3003184
PMID: 3003184
Abstract
The periodate-thiocarbohydrazide silver proteinate (PA-TCH-SP) method was used to study the envelopment process in varicella-zoster virus-infected human melanoma cells. Viral envelopment could be seen at two sites, the nuclear membrane and at virus-induced intracytoplasmic vacuoles. Virus-associated glycoconjugates were detected by the PA-TCH-SP method at the plasmalemma and on the inner membrane of the intracytoplasmic vacuoles. Virion envelopes acquired at the nuclear membrane were PA-TCH-SP negative, whereas those acquired at intracytoplasmic vacuoles were PA-TCH-SP positive. All virions found inside these vacuoles contained periodate-reactive envelopes. Release of virions into the extracellular space, where virtually all virions were PA-TCH-SP positive, appeared to be via exocytosis. Thus, the PA-TCH-SP method identifies glycoprotein incorporation at specific cytoplasmic vacuoles distinct from nuclear envelope, endoplasmic reticulum, and Golgi lamellae. These results suggest that envelopment within the cytoplasm is a stage in the assembly of the varicella-zoster virion.
Details
- Title: Subtitle
- Varicella-zoster viral glycoprotein envelopment: ultrastructural cytochemical localization
- Creators
- Eduardo A Montalvo - Univ. Texas, health sci. centerRichard T Parmley - Univ. Texas, health sci. centerCharles Grose - Univ. Texas, health sci. center
- Resource Type
- Journal article
- Publication Details
- The journal of histochemistry and cytochemistry, Vol.34(2), pp.281-284
- Publisher
- Histochemical Society
- DOI
- 10.1177/34.2.3003184
- PMID
- 3003184
- ISSN
- 0022-1554
- eISSN
- 1551-5044
- Language
- English
- Date published
- 1986
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093456202771
Metrics
17 Record Views