Varicella-zoster virus DNA polymerase and major DNA-binding protein genes have overlapping divergent promoters

Jeffery L Meier; Stephen E Straus

doi:10.1128/JVI.67.12.7573-7581.1993

Back

Varicella-zoster virus DNA polymerase and major DNA-binding protein genes have overlapping divergent promoters

Journal article

Open access

Peer reviewed

Varicella-zoster virus DNA polymerase and major DNA-binding protein genes have overlapping divergent promoters

Jeffery L Meier and Stephen E Straus

Journal of virology, Vol.67(12), pp.7573-7581

12/1993

DOI: 10.1128/JVI.67.12.7573-7581.1993

PMCID: PMC238224

PMID: 8230477

Files and links (1)

url

https://doi.org/10.1128/JVI.67.12.7573-7581.1993View

Published (Version of record) Open Access

Abstract

A detailed analysis of the transcriptionally divergent promoters of varicella-zoster virus (VZV) open reading frames (ORFs) 28 and 29, encoding the DNA polymerase and major DNA-binding proteins, respectively, was performed. We found that the 221-bp ORF 28-29 intergenic domain contains overlapping divergent promoters; these promoters have TATA boxes and cap sites arranged closely back-to-back, have highly concordant patterns of responsiveness to transactivation by VZV ORFs 4 and/or 62, and could not be separated without abolishing the effects that VZV trans activators imparted to them. Mutation of the ORF 28 TATA box rendered this promoter unresponsive to ORF 62 and the combination of ORFs 4 and 62 without altering ORF 29 promoter activity. Mutations of all potential ORF 29 TATA boxes collectively failed to abolish this promoter's responsiveness to either ORF 4 or ORF 62, suggesting a mechanism of gene regulation for ORF 29 that differs from that of ORF 28. These findings are concordant with the observation that both genes are expressed in productive infection, but only ORF 29 expression has been identified in latency.

Transfection

Consensus Sequence

Gene Expression Regulation, Viral - drug effects

Gene Expression - drug effects

Humans

Cells, Cultured

Chloramphenicol O-Acetyltransferase

Molecular Sequence Data

DNA-Binding Proteins - genetics

Promoter Regions, Genetic - genetics

DNA-Directed DNA Polymerase - genetics

Herpesvirus 3, Human - genetics

Animals

DNA Mutational Analysis

Base Sequence

Genes, Viral

TATA Box

Trans-Activators - pharmacology

Herpesvirus 3, Human - enzymology

Genes, Reporter

Details

Title: Subtitle: Varicella-zoster virus DNA polymerase and major DNA-binding protein genes have overlapping divergent promoters
Creators: Jeffery L Meier - Medical Virology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892
Stephen E Straus
Resource Type: Journal article
Publication Details: Journal of virology, Vol.67(12), pp.7573-7581
DOI: 10.1128/JVI.67.12.7573-7581.1993
PMID: 8230477
PMCID: PMC238224
ISSN: 0022-538X
eISSN: 1098-5514
Language: English
Date published: 12/1993
Academic Unit: Infectious Diseases; Epidemiology; Internal Medicine
Record Identifier: 9984094487202771

Metrics

12 Record Views

25 Times Cited - Web of Science

See more details