Journal article
Varicella zoster virus glycoprotein gpI is selectively phosphorylated by a virus-induced protein kinase
Proceedings of the National Academy of Sciences - PNAS, Vol.83(23), pp.8967-8971
1986
DOI: 10.1073/pnas.83.23.8967
PMCID: PMC387055
PMID: 3024158
Abstract
Varicella zoster virus glycoprotein I (VZV gpI; Mr 98,000) was phosphorylated in virus-infected human cell monolayers, while two other major VZV glycoproteins (gpII and gpIII) were not similarly modified. Phosphorylation of VZV gpI was not blocked by inhibitors of glycosylation, nor were the phosphoryl groups enzymatically removed by endoglycosidases. Phosphoamino acid analysis revealed the presence of phosphoserine and phosphothreonine residues on the polypeptide backbone. The selective nature of the phosphorylation event was further demonstrated in vitro by a protein kinase (Mr 50,000), which was present in virus-infected cells but absent from uninfected cells or purified virions. The enzyme catalyzed the transfer of 32Pi from [gamma-32P]ATP to gpI but not to gpII and gpIII. Like VZV gpI, this virus-induced protein kinase was also a constituent of the plasma membrane of live VZV-infected cells.
Details
- Title: Subtitle
- Varicella zoster virus glycoprotein gpI is selectively phosphorylated by a virus-induced protein kinase
- Creators
- E. A MONTALVO - Univ. Iowa, dep. microbiology pediatrics, Iowa City IA 52242, United StatesC GROSE - Univ. Iowa, dep. microbiology pediatrics, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.83(23), pp.8967-8971
- DOI
- 10.1073/pnas.83.23.8967
- PMID
- 3024158
- PMCID
- PMC387055
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 1986
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093310102771
Metrics
20 Record Views