Journal article
Varicella-zoster virus infection of diploid and chemically transformed guinea-pig embryo cells: factors influencing virus replication
Journal of general virology, Vol.54(Pt 2), pp.403-407
06/1981
DOI: 10.1099/0022-1317-54-2-403
PMID: 6270257
Abstract
Factors influencing the replication of varicella-zoster virus (VZV) in guinea-pig embryo cells were evaluated using both diploid cells (GPEC) and a chemically transformed cell line (GPT). Wild-type and vaccine strains of VZV were successfully isolated and serially propagated in GPEC prepared from early gestation whole embryos (less than 2 cm in length). Low passage GPEC (less than or equal to 5 subcultivations) were more susceptible to VZV infection than high passage GPEC (greater than 5 subcultivations), and guinea-pig cells were consistently less permissive than human diploid cells. Cell-free virus was produced from VZV-infected GPEC cultures by sonication and peak yields of 10(3) p.f.u./ml were obtained. In addition, we report the isolation and propagation of VZV, as well as production of cell-free virus, in GPT. Both GPEC and GPT cells were less susceptible to VZV infection than human cells. However, viral replication was enhanced by incubation of VZV-infected GPT cultures at 32 degree C rather than 36 degree C.
Details
- Title: Subtitle
- Varicella-zoster virus infection of diploid and chemically transformed guinea-pig embryo cells: factors influencing virus replication
- Creators
- Betty J EdmondCharles GrosePhilip A Brunell
- Resource Type
- Journal article
- Publication Details
- Journal of general virology, Vol.54(Pt 2), pp.403-407
- DOI
- 10.1099/0022-1317-54-2-403
- PMID
- 6270257
- NLM abbreviation
- J Gen Virol
- ISSN
- 0022-1317
- eISSN
- 1465-2099
- Grant note
- 14604 / PHS HHS
- Language
- English
- Date published
- 06/1981
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093344402771
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