Varicella-zoster virus-specific gp140: a highly immunogenic and disulfide-linked structural glycoprotein

C GROSE; D. P EDWARDS; K. A WEIGLE; W. E FRIEDRICHS; W. L MCGUIRE

doi:10.1016/0042-6822(84)90098-9

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Varicella-zoster virus-specific gp140: a highly immunogenic and disulfide-linked structural glycoprotein

Journal article

Peer reviewed

Varicella-zoster virus-specific gp140: a highly immunogenic and disulfide-linked structural glycoprotein

C GROSE, D. P EDWARDS, K. A WEIGLE, W. E FRIEDRICHS and W. L MCGUIRE

Virology (New York, N.Y.), Vol.132(1), pp.138-146

1984

DOI: 10.1016/0042-6822(84)90098-9

PMID: 6320530

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Abstract

A 140,000-dalton disulfide-linked glycoprotein (gp140) specified by varicella-zoster virus (VZV) in infected cultured cells was identified and precipitated by two murine monoclonal antibodies (VZ-151 and VZ-158). When analyzed under reducing conditions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gp140 was cleaved predominantly into a 66,000 lower-molecular-weight product (gp66). This protein was classified as a viral structural component, since it was observed in the polypeptide profile of metrizamide gradient-purified enveloped virions. By immunofluorescence analyses with a monoclonal antibody probe, gp140 expression was documented to be highly conserved both within cultured cells inoculated with homologous (VZV-Oka) and heterologous (VZV-32) strains and in infected human tissues from chicken pox and zoster patients. That the glycoprotein was highly immunogenic was confirmed by the presence of high-titer anti-gp140 antibody in the sera of both hyperimmunized laboratory animals and naturally infected humans. Temporally, the humoral response to gp140 following primary VZV infection preceded that against the other viral glycoproteins. These studies describe, therefore, an immunogenic, disulfide-linked viral structural glycoprotein, which must be included among the other five previously described VZV-specific fucosylated species--gp118, gp98, gp62, and gp45.

Microbiology

Virology

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Morphology, structure, chemical composition, physicochemical properties

Details

Title: Subtitle: Varicella-zoster virus-specific gp140: a highly immunogenic and disulfide-linked structural glycoprotein
Creators: C GROSE - Univ. Texas health sci. center
D. P EDWARDS - Univ. Texas health sci. center
K. A WEIGLE - Univ. Texas health sci. center
W. E FRIEDRICHS - Univ. Texas health sci. center
W. L MCGUIRE - Univ. Texas health sci. center
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.132(1), pp.138-146
DOI: 10.1016/0042-6822(84)90098-9
PMID: 6320530
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier
Language: English
Date published: 1984
Academic Unit: Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
Record Identifier: 9984093595602771

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