Journal article
Vascular Smooth Muscle Cells Are Responsible for a Prothrombotic Phenotype of Spontaneously Hypertensive Rat Arteries
Arteriosclerosis, thrombosis, and vascular biology, Vol.35(4), pp.930-937
04/2015
DOI: 10.1161/ATVBAHA.115.305377
PMID: 25722431
Abstract
Objective-The hypothesis that hypertension induces a hypercoagulable state arises from the complications associated with hypertension: stroke and myocardial infarction. Here, we determine whether hypertension causes changes in the thrombin-generating capacity of the vascular wall. Approach and Results-We used spontaneously hypertensive rats (SHR) compared with Wistar rats. The addition of thoracic aortic rings of SHR to a Wistar or SHR plasma pool resulted in a greater increase in thrombin generation compared with equivalent rings from Wistar. This increase occurred in 12-but not 5-week-old rats and was prevented by an angiotensin II-converting enzyme inhibitor, indicating that established hypertension is required to induce increased thrombin generation within the vessel wall. Whereas no difference was observed for endothelial cells, thrombin formation was higher on aortic smooth muscle cells (SMCs) from SHR than on those from Wistar. Exposure of negatively charged phospholipids was higher on SHR than on Wistar rings, as well as on cultured SMCs. Tissue factor activity was higher in SHR SMCs. Twelve-week-old SHR exhibited accelerated FeCl3-induced thrombus formation in carotid arteries, and the resulting occlusive thrombi were disaggregated by blockade of glycoprotein Iba-von Willebrand factor interactions. SHR SMCs were more sensitive to thrombin-induced proliferation than Wistar SMCs. This effect was totally abolished by a protease-activated receptor 1 inhibitor. Conclusions-The prothrombotic phenotype of the SHR vessel wall was due to the ability of SMCs to support greater thrombin generation and resulted in accelerated occlusive thrombus formation after arterial injury, which was sensitive to glycoprotein Iba-von Willebrand factor inhibitors.
Details
- Title: Subtitle
- Vascular Smooth Muscle Cells Are Responsible for a Prothrombotic Phenotype of Spontaneously Hypertensive Rat Arteries
- Creators
- Karima Ait Aissa - Défaillance Cardiovasculaire Aiguë et ChroniqueJeremy Lagrange - Défaillance Cardiovasculaire Aiguë et ChroniqueAmel Mohamadi - Défaillance Cardiovasculaire Aiguë et ChroniqueHuguette Louis - Défaillance Cardiovasculaire Aiguë et ChroniqueBenedicte Houppert - Défaillance Cardiovasculaire Aiguë et ChroniquePascal Challande - Modélisation, Propagation et Imagerie AcoustiqueDenis Wahl - Défaillance Cardiovasculaire Aiguë et ChroniquePatrick Lacolley - Défaillance Cardiovasculaire Aiguë et ChroniqueVeronique Regnault - Défaillance Cardiovasculaire Aiguë et Chronique
- Resource Type
- Journal article
- Publication Details
- Arteriosclerosis, thrombosis, and vascular biology, Vol.35(4), pp.930-937
- Publisher
- American Heart Association
- DOI
- 10.1161/ATVBAHA.115.305377
- PMID
- 25722431
- ISSN
- 1079-5642
- eISSN
- 1524-4636
- Language
- English
- Date published
- 04/2015
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984359564602771
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