Journal article
Vascular cell adhesion molecule-1 augments adenovirus-mediated gene transfer
Arteriosclerosis, thrombosis, and vascular biology, Vol.21(2), pp.238-242
2001
DOI: 10.1161/01.ATV.21.2.238
PMID: 11156859
Abstract
We have reported that adenovirus-mediated gene transfer is augmented in the endothelium of atherosclerotic blood vessels. We observed that vascular cell adhesion molecule-1 (VCAM-1) shares some homology with the coxsackievirus and adenovirus receptor. Because VCAM-1 is upregulated on atherosclerotic endothelial cells, we hypothesized that VCAM-1 may act as an auxiliary receptor to augment adenovirus-mediated gene transfer. To test this hypothesis, stable NIH 3T3 cell lines that constitutively express VCAM-1 on the cell surface were generated. Recombinant adenovirus 5 (Ad5), which contains the reporter β-galactosidase gene, was used to compare Ad5 infection in VCAM-1+ and parental NIH 3T3 cells. Total β-galactosidase activity and the number of transgene-positive cells were 6- to 10-fold and 5-fold higher, respectively, in VCAM-1+ than in VCAM-1− cells. Ad5 binding to VCAM-1+ cells was increased by 3-fold over VCAM-1− cells. Soluble VCAM-1 protein, present during infection or viral binding, reduced β-galactosidase activity in VCAM-1+ cells in a dose-dependent manner. Taken together, we conclude that VCAM-1 can mediate adenovirus binding and infection. This may explain, in part, the previous finding that adenovirus-mediated gene transfer is augmented in atherosclerotic arteries.
Details
- Title: Subtitle
- Vascular cell adhesion molecule-1 augments adenovirus-mediated gene transfer
- Creators
- Yi Chu - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, United StatesDonald D HEISTAD - Department of Pharmacology, University of Iowa College of Medicine, Iowa City, United StatesMyron I CYBULSKY - Department of Laboratory Medicine and Pathobiology, The Toronto Hospital, University of Toronto, Toronto, Ontario, CanadaBeverly L DAVIDSON - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, United States
- Resource Type
- Journal article
- Publication Details
- Arteriosclerosis, thrombosis, and vascular biology, Vol.21(2), pp.238-242
- DOI
- 10.1161/01.ATV.21.2.238
- PMID
- 11156859
- NLM abbreviation
- Arterioscler Thromb Vasc Biol
- ISSN
- 1079-5642
- eISSN
- 1524-4636
- Publisher
- Lippincott; Philadelphia, PA; Hagerstown, MD
- Language
- English
- Date published
- 2001
- Academic Unit
- Cardiovascular Medicine; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040528802771
Metrics
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