Journal article
Vasoactive intestinal peptide stimulates androgen biosynthesis by cultured neonatal testicular cells
Molecular and cellular endocrinology, Vol.48(1), pp.21-29
1986
DOI: 10.1016/0303-7207(86)90162-0
PMID: 2430845
Abstract
Vasoactive intestinal peptide (VIP) was originally isolated from porcine duodenum and considered to be a gut hormone. Recent evidence indicates that it may also be involved in reproductive functions. In this study, a possible action of VIP on steroidogenesis by cultured testicular cells was investigated. Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay. Treatment of cultured cells with VIP (10
−9 to 10
−6 M) increased the production of testosterone, progesterone, and pregnenolone in a dose-dependent fashion. Testosterone production in response to 10
−6 M VIP was about 5–10% of that maximally induced by LH. Addition of methyl-isobutyl-xanthine, a phosphodiesterase inhibitor, to the VIP-containing cultures significantly enhanced production of testosterone by 13-fold, of progesterone by 9-fold, and of pregnenolone by 2.5-fold as compared to treatment with VIP alone. Additional experiments also showed a dose-dependent stimulation of cAMP production by VIP. The VIP-related hormones PHM-27, secretin, and glucagon also stimulated progesterone and testosterone production with a potency order (PHM-27 > secretin > glucagon) consistent with that observed for other VIP receptor-mediated actions. A direct stimulatory effect of VIP on Leydig cells was indicated in studies on steroidogenesis by testicular cells separated on a metrizamide density gradient. In these studies, VIP stimulated androgen production in an LH-responsive subpopulation of testis cells but failed to affect steroid production in non-LH-responsive cells. Thus, these experiments demonstrate that VIP, independent of LH, stimulates steroidogenesis in cultured neonatal testicular cells and suggest a possible stimulatory role for VIP in testicular steroidogenesis by the developing testis.
Details
- Title: Subtitle
- Vasoactive intestinal peptide stimulates androgen biosynthesis by cultured neonatal testicular cells
- Creators
- Barry G. Kasson - University of California, San DiegoPaul Lim - University of California, San DiegoAaron J.W. Hsueh - University of California, San Diego
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular endocrinology, Vol.48(1), pp.21-29
- Publisher
- Elsevier Ireland Ltd
- DOI
- 10.1016/0303-7207(86)90162-0
- PMID
- 2430845
- ISSN
- 0303-7207
- eISSN
- 1872-8057
- Language
- English
- Date published
- 1986
- Academic Unit
- Neuroscience and Pharmacology
- Record Identifier
- 9984303747802771
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