Journal article
Vasodilator actions of the endothelium-derived relaxing factor l-S-nitrosocysteine in anaesthetized rats are markedly diminished by peroxynitrite
Clinical and experimental pharmacology & physiology, Vol.32(12), pp.1137-1141
12/2005
DOI: 10.1111/j.1440-1681.2005.04310.x
PMID: 16445582
Abstract
Summary
1. The aim of the present study was to assess the effects of the potent oxidant and nitrating agent peroxynitrite on the haemodynamic actions of the endothelium-derived S-nitrosothiol l-S-nitrosocysteine.
2. The haemodynamic actions of l-S-nitrosocysteine (12.5–100 nmol/kg, i.v.) were determined in pentobarbital-anaesthetized rats before and after the induction of tachyphylaxis to peroxynitrite achieved by giving 10 intravenous injections of a 10 µmol/kg dose.
3. l-S-Nitrosocysteine elicited dose-dependent reductions in mean arterial blood pressure and in hindquarter and mesenteric vascular resistance.
4. The l-S-nitrosocysteine-induced responses were substantially attenuated after administration of peroxynitrite.
5. We have reported previously that nitric oxide-mediated vasodilation is not diminished after the induction of tachyphylaxis to peroxynitrite. Taken together, these findings support the concept that peroxynitrite reduces the vasodilator actions of l-S-nitrosocysteine via oxidation and/or nitration of putative membrane-bound S-nitrosothiol recognition sites.
Details
- Title: Subtitle
- Vasodilator actions of the endothelium-derived relaxing factor l-S-nitrosocysteine in anaesthetized rats are markedly diminished by peroxynitrite
- Creators
- Jonathan E Graves - Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GeorgiaJames N Bates - University of Iowa, AnesthesiaNeil W Kooy - Department of Pediatrics, Division of Critical Care Medicine, Children's Hospital Medical Center, Cincinnati, Ohio, USAStephen J Lewis - Case Western Reserve University
- Resource Type
- Journal article
- Publication Details
- Clinical and experimental pharmacology & physiology, Vol.32(12), pp.1137-1141
- Publisher
- Blackwell Science Pty
- DOI
- 10.1111/j.1440-1681.2005.04310.x
- PMID
- 16445582
- ISSN
- 0305-1870
- eISSN
- 1440-1681
- Number of pages
- 5
- Language
- English
- Date published
- 12/2005
- Academic Unit
- Anesthesia
- Record Identifier
- 9984424842902771
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