Journal article
Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein
Developmental biology, Vol.411(1), pp.115-127
03/01/2016
DOI: 10.1016/j.ydbio.2016.01.002
PMCID: PMC4769896
PMID: 26769101
Abstract
The mechanisms underlying organ vascularization are not well understood. The zebrafish intestinal vasculature forms early, is easily imaged using transgenic lines and in-situ hybridization, and develops in a stereotypical pattern thus making it an excellent model for investigating mechanisms of organ specific vascularization. Here, we demonstrate that the sub-intestinal vein (SIV) and supra-intestinal artery (SIA) form by a novel mechanism from angioblasts that migrate out of the posterior cardinal vein and coalesce to form the intestinal vasculature in an anterior to posterior wave with the SIA forming after the SIV. We show that vascular endothelial growth factor as (vegfaa) is expressed in the endoderm at the site where intestinal vessels form and therefore likely provides a guidance signal. Vegfa/Vegfr2 signaling is required for early intestinal vasculature development with mutation in vegfaa or loss of Vegfr2 homologs causing nearly complete inhibition of the formation of the intestinal vasculature. Vegfc and Vegfr3 function, however, are dispensable for intestinal vascularization. Interestingly, ubiquitous overexpression of Vegfc resulted in an overgrowth of the Sly, suggesting that Vegfc is sufficient to induce SIV development. These results argue that Vegfa signaling directs endothelial cells to migrate out of existing vasculature and coalesce to form the intestinal vessels. It is likely that a similar mechanism is utilized during vascularization of other organs. (C) 2016 Elsevier Inc. All rights reserved.
Details
- Title: Subtitle
- Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein
- Creators
- Andrew L. Koenig - Cincinnati Children's Hospital Medical CenterKristina Baltrunaite - Cincinnati Children's Hospital Medical CenterNeil I. Bower - The University of QueenslandAndrea Rossi - Max Planck Institute for Heart and Lung ResearchDidier Y. R. Stainier - Max Planck Institute for Heart and Lung ResearchBenjamin M. Hogan - The University of QueenslandSaulius Sumanas - Cincinnati Children's Hospital Medical Center
- Resource Type
- Journal article
- Publication Details
- Developmental biology, Vol.411(1), pp.115-127
- DOI
- 10.1016/j.ydbio.2016.01.002
- PMID
- 26769101
- PMCID
- PMC4769896
- NLM abbreviation
- Dev Biol
- ISSN
- 0012-1606
- eISSN
- 1095-564X
- Publisher
- Elsevier
- Number of pages
- 13
- Grant note
- Max Planck Society T32HL125204 / National Heart Lung and Blood Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) R01 HL107369; T32 HL125204 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 03/01/2016
- Academic Unit
- Stead Family Department of Pediatrics
- Record Identifier
- 9985161456502771
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