Journal article
Ventilator-associated lung injury decreases lung ability to clear edema in rats
American journal of respiratory and critical care medicine, Vol.159(2), pp.603-609
1999
DOI: 10.1164/ajrccm.159.2.9805050
PMID: 9927379
Abstract
Ventilator-associated lung injury (VALI) is caused by high tidal volume (Vt) excursions producing microvascular leakage and pulmonary edema. However, the effects of VALI on lung edema clearance and alveolar epithelial cells' Na,K-ATPase function have not been elucidated. We studied lung edema clearance in the isolated–perfused rat lung model after ventilation for 25, 40, and 60 min with high Vt (peak airway opening pressure [Pao] of approximately 35 cm H2O) and compared them with low Vt ventilation (Pao ∼ 8 cm H2O), moderate Vt ventilation (Pao ∼ 20 cm H2O), and nonventilated rats. Lung edema clearance in control rats was 0.50 ± 0.02 ml/h and decreased after 40 and 60 min of high Vt to 0.26 ± 0.03 and 0.11 ± 0.08 ml/h, respectively (p < 0.01), but did not change after low Vt and moderate Vt ventilation at any time point. Lung permeability to small (22Na+, [3H]mannitol) and large solutes (fluorescein isothiocyanate–tagged albumin [FITC–albumin]) increased significantly in rats ventilated for 60 min with high Vt, compared with low Vt, moderate Vt, and control rats (p < 0.01). Paralleling the impairment in lung edema clearance we found a decrease in Na,K-ATPase activity in alveolar type II (ATII) cells isolated from rats ventilated with moderate Vt and high Vt for 40 min without changes in α 1 Na,K-ATPase mRNA. We reason that VALI decreases lung ability to clear edema by inhibiting active sodium transport and Na,K-ATPase function in the alveolar epithelium.
Details
- Title: Subtitle
- Ventilator-associated lung injury decreases lung ability to clear edema in rats
- Creators
- E Lecuona - University of Illinois at ChicagoF Saldias - Pontificia Universidad Católica de ChileA Comellas - Pontificia Universidad Católica de ChileK Ridge - Pontificia Universidad Católica de ChileC Guerrero - Pontificia Universidad Católica de ChileJ. I Sznajder - Pontificia Universidad Católica de Chile
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory and critical care medicine, Vol.159(2), pp.603-609
- Publisher
- American Lung Association
- DOI
- 10.1164/ajrccm.159.2.9805050
- PMID
- 9927379
- ISSN
- 1073-449X
- eISSN
- 1535-4970
- Language
- English
- Date published
- 1999
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; ICTS; Internal Medicine
- Record Identifier
- 9984359764902771
Metrics
28 Record Views