Journal article
Vertical transmission of X4-tropic and dual-tropic HIV-1 in five Ugandan mother–infant pairs
AIDS (London), Vol.23(14), pp.1903-1908
09/10/2009
DOI: 10.1097/QAD.0b013e32832f1802
PMCID: PMC2764460
PMID: 19593079
Abstract
Background: We previously reported the existence of CXCR4-using HIV-1 in 6-14 week-old Ugandan infants. Whether these viruses were transmitted from the mother perinatally or evolved after transmission is not known. In the current study, we investigated the origin of the CXCR4-using viruses in these infants by comparing HIV-1 envelope clones from the infants to those from their mothers at or near the time of delivery.
Methods: Envelope clones were isolated from five Ugandan infant plasma samples that harbored CXCR4-using viruses, collected at the time of HIV diagnosis (four at birth, one at week 6), and from their mothers at delivery. Coreceptor usage and phylogenetic relatedness of HIV-1 populations in mother-infant pairs were analyzed in detail using the Trofile assay and sequence analysis of envelope clones, respectively.
Results: X4-tropic clones were identified in two mother-infant pairs and dual-tropic clones were found in three pairs, either alone or in combination with R5-tropic viruses. Dual-tropic clones varied in their ability to infect CXCR4-expressing cells. In each mother-infant pair, X4-tropic or dual-tropic clones shared similar phenotypic profiles and V3 sequence patterns; gp160 sequences of X4-tropic and dual-tropic clones from infants were phylogenetically indistinguishable from those of their mothers. The virus populations were phylogenetically homogenous in three infants and segregated according to coreceptor tropism in the remaining two infants.
Conclusions: This study demonstrates that X4-tropic and dual-tropic HIV-1 can be transmitted from mother to infant, before, during or shortly after delivery, and establishes vertical transmission as an important source of CXCR4-using viruses in infants.
Details
- Title: Subtitle
- Vertical transmission of X4-tropic and dual-tropic HIV-1 in five Ugandan mother–infant pairs
- Creators
- Wei Huang - Monogram Biosciences, South San Francisco, CaliforniaSusan H Eshleman - Johns Hopkins University School of Medicine, Baltimore, Maryland, USAJonathan Toma - Monogram Biosciences, South San Francisco, CaliforniaEric Stawiski - Monogram Biosciences, South San Francisco, CaliforniaJeannette M Whitcomb - Monogram Biosciences, South San Francisco, CaliforniaJ. Brooks Jackson - Johns Hopkins University School of Medicine, Baltimore, Maryland, USALaura Guay - Johns Hopkins University School of Medicine, Baltimore, Maryland, USAPhilippa Musoke - Makerere University School of Medicine, Kampala, UgandaNeil Parkin - Monogram Biosciences, South San Francisco, CaliforniaChristos J Petropoulos - Monogram Biosciences, South San Francisco, California
- Resource Type
- Journal article
- Publication Details
- AIDS (London), Vol.23(14), pp.1903-1908
- DOI
- 10.1097/QAD.0b013e32832f1802
- PMID
- 19593079
- PMCID
- PMC2764460
- NLM abbreviation
- AIDS
- ISSN
- 0269-9370
- eISSN
- 1473-5571
- Grant note
- U01 AI068613-04 || AI / National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID R43 AI048990 || AI / National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID U01 AI068632-03 || AI / National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID
- Language
- English
- Date published
- 09/10/2009
- Academic Unit
- Pathology; VPMA - Administration
- Record Identifier
- 9984046900802771
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