Very diverse CD8 T cell clonotypic responses after virus infections

Lecia L Pewe; Jason M Netland; Stephen B Heard; Stanley Perlman

doi:10.4049/jimmunol.172.5.3151

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Very diverse CD8 T cell clonotypic responses after virus infections

Journal article

Peer reviewed

Very diverse CD8 T cell clonotypic responses after virus infections

Lecia L Pewe, Jason M Netland, Stephen B Heard and Stanley Perlman

Journal of immunology (Baltimore, Md. : 1950), Vol.172(5), pp.3151-3156

03/01/2004

DOI: 10.4049/jimmunol.172.5.3151

PMID: 14978121

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Abstract

We measured CD8 T cell clonotypic diversity to three epitopes recognized in C57BL/6 mice infected with mouse hepatitis virus, strain JHM, or lymphocytic choriomeningitis virus. We isolated epitope-specific T cells with an IFN-gamma capture assay or MHC class I/peptide tetramers and identified different clonotypes by Vbeta chain sequence analysis. In agreement with our previous results, the number of different clonotypes responding to all three epitopes fit a log-series distribution. From these distributions, we estimated that >1000 different clonotypes responded to each immunodominant CD8 T cell epitope; the response to a subdominant CD8 T cell epitope was modestly less diverse. These results suggest that T cell response diversity is greater by 1-2 orders of magnitude than predicted previously.

Spleen - immunology

Lymphocyte Activation

Mice, Inbred C57BL

Viral Proteins - immunology

Spleen - cytology

Gene Rearrangement, beta-Chain T-Cell Antigen Receptor

Coronavirus Infections - immunology

Receptors, Antigen, T-Cell, alpha-beta - genetics

Murine hepatitis virus - immunology

Animals

Coronavirus Infections - metabolism

Lymphocyte Count - statistics & numerical data

Spleen - metabolism

CD8-Positive T-Lymphocytes - virology

Peptide Fragments - immunology

Receptors, Antigen, T-Cell, alpha-beta - biosynthesis

CD8-Positive T-Lymphocytes - metabolism

Poisson Distribution

Clone Cells

Mice

Epitopes, T-Lymphocyte - immunology

CD8-Positive T-Lymphocytes - immunology

Lymphocyte Count - methods

Details

Title: Subtitle: Very diverse CD8 T cell clonotypic responses after virus infections
Creators: Lecia L Pewe - Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA
Jason M Netland
Stephen B Heard
Stanley Perlman
Resource Type: Journal article
Publication Details: Journal of immunology (Baltimore, Md. : 1950), Vol.172(5), pp.3151-3156
DOI: 10.4049/jimmunol.172.5.3151
PMID: 14978121
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: NS40438 / NINDS NIH HHS
Language: English
Date published: 03/01/2004
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777347102771

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