Journal article
VikAD, a Vika site-specific recombinase-based system for efficient and scalable helper-dependent adenovirus production
Molecular therapy. Methods & clinical development, Vol.24, pp.117-126
12/2021
DOI: 10.1016/j.omtm.2021.12.001
PMCID: PMC8718833
PMID: 35024378
Abstract
Recombinant viral vectors have become integral tools for basic in vivo research applications. Helper-dependent adenoviral (HdAd) vectors have a large packaging capacity of ∼36 kb of DNA that mediate long-term transgene expression in vitro and in vivo. The large carrying capacity of HdAd enables basic research or clinical applications requiring the delivery of large genes or multiple transgenes which cannot be packaged into other widely-used viral vectors. Currently, common HdAd production systems use a helper Ad virus (HV) with a packaging signal (Ψ) that is flanked by either loxP or FRT sites, which is excised in producer cells expressing Cre or Flp recombinases to prevent HV packaging. However, these production systems prevent the use of HdAd vectors for genetic strategies that rely on Cre or Flp recombination for cell-type specific expression. To overcome these limitations, we developed the VikAD production system, which is based on producer cells expressing the Vika recombinase and a HV that contains a Ψ flanked by vox sites. The availability of this production system will greatly expand the utility and flexibility of HdAd vectors for use in research applications to monitor and manipulate cellular activity with increased specificity.
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VikAD is a Helper-Dependent Adenovirus (HdAd) production platform that utilizes a cell line expressing the Vika site-specific recombinase and an Ad Helper Virus that contains the packaging sequence flanked by vox sites. VikAD enables use of HdAd with genetic intersectional strategies using Cre and Flp or other site-specific recombinases.
Details
- Title: Subtitle
- VikAD, a Vika site-specific recombinase-based system for efficient and scalable helper-dependent adenovirus production
- Creators
- Stacia Phillips - Department of Anatomy and Cell BiologyPaula Valino Ramos - Department of Anatomy and Cell BiologyPriyadharishini Veeraraghavan - Department of Anatomy and Cell BiologySamuel M Young - Department of Anatomy and Cell Biology
- Resource Type
- Journal article
- Publication Details
- Molecular therapy. Methods & clinical development, Vol.24, pp.117-126
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.omtm.2021.12.001
- PMID
- 35024378
- PMCID
- PMC8718833
- ISSN
- 2329-0501
- eISSN
- 2329-0501
- Grant note
- DOI: 10.13039/100000055, name: National Institute on Deafness and Other Communication Disorders; DOI: 10.13039/100000065, name: National Institute of Neurological Disorders and Stroke; DOI: 10.13039/100008893, name: University of Iowa
- Language
- English
- Date published
- 12/2021
- Academic Unit
- Microbiology and Immunology; Anatomy and Cell Biology; Iowa Neuroscience Institute; Otolaryngology
- Record Identifier
- 9984201784002771
Metrics
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