Vinculin-dependent actin bundling regulates cell migration and traction forces

Karry M Jannie; Shawn M Ellerbroek; Dennis W Zhou; Sophia Chen; David J Crompton; Andrés J García; Kris A DeMali

doi:10.1042/BJ20140872

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Vinculin-dependent actin bundling regulates cell migration and traction forces

Journal article

Peer reviewed

Vinculin-dependent actin bundling regulates cell migration and traction forces

Karry M Jannie, Shawn M Ellerbroek, Dennis W Zhou, Sophia Chen, David J Crompton, Andrés J García and Kris A DeMali

Biochemical journal, Vol.465(3), pp.383-393

02/01/2015

DOI: 10.1042/BJ20140872

PMCID: PMC4418518

PMID: 25358683

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Abstract

Vinculin binding to actin filaments is thought to be critical for force transduction within a cell, but direct experimental evidence to support this conclusion has been limited. In the present study, we found mutation (R1049E) of the vinculin tail impairs its ability to bind F-actin, stimulate actin polymerization, and bundle F-actin in vitro. Further, mutant (R1049E) vinculin expressing cells are altered in cell migration, which is accompanied by changes in cell adhesion, cell spreading and cell generation of traction forces, providing direct evidence for the critical role of vinculin in mechanotransduction at adhesion sites. Lastly, we discuss the viability of models detailing the F-actin-binding surface on vinculin in the context of our mutational analysis.

Actin Cytoskeleton - metabolism

Protein Structure, Secondary

Actins - metabolism

Vinculin - metabolism

Actin Cytoskeleton - chemistry

Mechanotransduction, Cellular - physiology

Cell Movement - physiology

Mice, Knockout

Vinculin - chemistry

Animals

Actins - chemistry

Mice

Protein Binding - physiology

Details

Title: Subtitle: Vinculin-dependent actin bundling regulates cell migration and traction forces
Creators: Karry M Jannie - Department of Biochemistry, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, U.S.A
Shawn M Ellerbroek - Department of Biochemistry, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, U.S.A
Dennis W Zhou - †Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, U.S.A
Sophia Chen - Department of Biochemistry, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, U.S.A
David J Crompton - Department of Biochemistry, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, U.S.A
Andrés J García - †Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, U.S.A
Kris A DeMali - Department of Biochemistry, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, U.S.A
Resource Type: Journal article
Publication Details: Biochemical journal, Vol.465(3), pp.383-393
Publisher: England
DOI: 10.1042/BJ20140872
PMID: 25358683
PMCID: PMC4418518
ISSN: 0264-6021
eISSN: 1470-8728
Grant note: R01 GM065918 / NIGMS NIH HHS GM065918 / NIGMS NIH HHS P30CA086862 / NCI NIH HHS P30 CA086862 / NCI NIH HHS
Language: English
Date published: 02/01/2015
Academic Unit: Dermatology; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology
Record Identifier: 9984024536402771

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