Journal article
Viral Infection Results in Massive CD8 + T Cell Expansion and Mortality in Vaccinated Perforin-Deficient Mice
Immunity (Cambridge, Mass.), Vol.18(4), pp.463-474
2003
DOI: 10.1016/S1074-7613(03)00079-7
PMID: 12705850
Abstract
Perforin-mediated cytotoxicity is essential for clearance of primary LCMV infection. BALB/c-perforin-deficient (PKO) mice survived LCMV infection by deleting NP
118-specific CD8
+ T cells whereas vaccination of PKO mice with
Listeria expressing NP
118 generated a stable memory CD8
+ T cell population. However, >85% of vaccinated BALB/c-PKO mice died after LCMV infection. Mortality was associated with enormous expansion of NP
118-specific CD8
+ T cells in both lymphoid and nonlymphoid tissues and aberrant CD8
+ T cell cytokine production. Depletion of CD8
+ T cells or treatment with anti-IFNγ antibody rescued vaccinated mice from mortality. Thus, perforin was essential for resistance to secondary LCMV infection, and, in the absence of perforin, vaccination resulted in lethal disease mediated by dysregulated CD8
+ T cell expansion and cytokine production.
Details
- Title: Subtitle
- Viral Infection Results in Massive CD8 + T Cell Expansion and Mortality in Vaccinated Perforin-Deficient Mice
- Creators
- Vladimir P Badovinac - Department of Microbiology, University of Iowa, Iowa City, IA 52242 USASara E Hamilton - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242 USAJohn T Harty - Department of Microbiology, University of Iowa, Iowa City, IA 52242 USA
- Resource Type
- Journal article
- Publication Details
- Immunity (Cambridge, Mass.), Vol.18(4), pp.463-474
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S1074-7613(03)00079-7
- PMID
- 12705850
- ISSN
- 1074-7613
- eISSN
- 1097-4180
- Language
- English
- Date published
- 2003
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984047601702771
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