Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease

Mathieu Garand; Susie S. Y. Huang; Lisa S. Goessling; Fei Wan; Donna A. Santillan; Mark K. Santillan; Anoop Brar; Todd N. Wylie; Kristine M. Wylie; Pirooz Eghtesady

doi:10.3390/microorganisms11020262

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Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease

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Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease

Mathieu Garand, Susie S. Y. Huang, Lisa S. Goessling, Fei Wan, Donna A. Santillan, Mark K. Santillan, Anoop Brar, Todd N. Wylie, Kristine M. Wylie and Pirooz Eghtesady

Microorganisms (Basel), Vol.11(2), 262

01/19/2023

DOI: 10.3390/microorganisms11020262

PMCID: PMC9963073

PMID: 36838226

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Abstract

Background: We have previously shown coxsackievirus B (CVB) to be a potent inducer of congenital heart disease (CHD) in mice. The clinical relevance of these findings in humans and the roles of other viruses in the pathogenesis of CHD remain unknown. Methods: We obtained plasma samples, collected at all trimesters, from 89 subjects (104 pregnancies), 73 healthy controls (88 pregnancies), and 16 with CHD–affected birth (16 pregnancies), from the Perinatal Family Tissue Bank (PFTB). We performed CVB IgG/IgM serological assays on plasma. We also used ViroCap sequencing and PCR to test for viral nucleic acid in plasma, circulating leukocytes from the buffy coat, and in the media of a co-culture system. Results: CVB IgG/IgM results indicated that prior exposure was 7.8 times more common in the CHD group (95% CI, 1.14–54.24, adj. p-value = 0.036). However, the CVB viral genome was not detected in plasma, buffy coat, or co-culture supernatant by molecular assays, although other viruses were detected. Conclusion: Detection of viral nucleic acid in plasma was infrequent and specifically no CVB genome was detected. However, serology demonstrated that prior CVB exposure is higher in CHD-affected pregnancies. Further studies are warranted to understand the magnitude of the contribution of the maternal blood virome to the pathogenesis of CHD.

Details

Title: Subtitle: Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart Disease
Creators: Mathieu Garand - Washington University in St. Louis
Susie S. Y. Huang
Lisa S. Goessling
Fei Wan
Donna A. Santillan
Mark K. Santillan
Anoop Brar
Todd N. Wylie
Kristine M. Wylie
Pirooz Eghtesady
Resource Type: Journal article
Publication Details: Microorganisms (Basel), Vol.11(2), 262
DOI: 10.3390/microorganisms11020262
PMID: 36838226
PMCID: PMC9963073
NLM abbreviation: Microorganisms
ISSN: 2076-2607
eISSN: 2076-2607
Grant note: DOI: 10.13039/100000968, name: American Heart Association, award: 19IPLOI34760405; DOI: 10.13039/100000002, name: National Institutes of Health, award: R01HD089940 P50HD103556-8719, NIH CTSA UL1TR002537; DOI: 10.13039/100011343, name: Holden Comprehensive Cancer Center, award: P30CA086862
Language: English
Date published: 01/19/2023
Academic Unit: Obstetrics and Gynecology
Record Identifier: 9984360124102771

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