Virulence of different Pseudomonas species in a burned mouse model: tissue colonization by Pseudomonas cepacia

G Byron Stover; David R Drake; Thomas C Montie

doi:10.1128/IAI.41.3.1099-1104.1983

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Virulence of different Pseudomonas species in a burned mouse model: tissue colonization by Pseudomonas cepacia

Journal article

Open access

Peer reviewed

Virulence of different Pseudomonas species in a burned mouse model: tissue colonization by Pseudomonas cepacia

G Byron Stover, David R Drake and Thomas C Montie

Infection and immunity, Vol.41(3), pp.1099-1104

1983

DOI: 10.1128/IAI.41.3.1099-1104.1983

PMCID: PMC264613

PMID: 6885156

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Abstract

The virulence of Pseudomonas aeruginosa and other pseudomonads was examined in a burned mouse model. P. aeruginosa M-2 was highly virulent causing 100% mortality by 38 h with an injection of 10(2) CFU by either a subcutaneous or intraperitoneal route. Subcutaneous injection of 10(2) CFU revealed rapid multiplication of the bacteria at the burn wound with 10(8) CFU/g detectable in the burned skin by 28 h postinjection, 10(5) CFU/g of liver, and 10(3) CFU/ml of blood. Non-P. aeruginosa clinical isolates were markedly less virulent; an injection of greater than or equal to 10(7) CFU caused less than or equal to 60% lethality. P. cepacia SMH colonized the burned skin of thermally injured mice, persisting at levels of 10(7) to 10(8) CFU/g of burned skin after an initial injection of 10(5) CFU. P. cepacia persisted in the burn wound for at least 3 weeks. No organ invasion was detectable throughout this period. Studies with an additional clinical isolate of P. cepacia yielded similar results. An injection of a 10(2) CFU dose revealed that the level of persistence is dose dependent. Results suggest that the tenacious persistence of P. cepacia in the burn wound may provide a model for the study of persistent colonization and infection in a compromised host.

Bacteriology

Microbiology

Fundamental and applied biological sciences. Psychology

Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains

Biological and medical sciences

Details

Title: Subtitle: Virulence of different Pseudomonas species in a burned mouse model: tissue colonization by Pseudomonas cepacia
Creators: G Byron Stover - Univ. Tennessee, dep. microbiology
David R Drake - Univ. Tennessee, dep. microbiology
Thomas C Montie - Univ. Tennessee, dep. microbiology
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.41(3), pp.1099-1104
DOI: 10.1128/IAI.41.3.1099-1104.1983
PMID: 6885156
PMCID: PMC264613
NLM abbreviation: Infect Immun
ISSN: 0019-9567
eISSN: 1098-5522
Publisher: American Society for Microbiology; Washington, DC
Language: English
Date published: 1983
Academic Unit: Endodontics; Dental Research
Record Identifier: 9984065976002771

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