Logo image
Back
Virus-free and oncogene-free induced pluripotent stem cell reprogramming in cord blood and peripheral blood in patients with lung disease
Journal article   Open access   Peer reviewed

Virus-free and oncogene-free induced pluripotent stem cell reprogramming in cord blood and peripheral blood in patients with lung disease

Anant Kamath, Sara Ternes, Stephen McGowan and Alan B. Moy
Regenerative medicine, Vol.13(8), pp.899-915
12/01/2018
DOI: 10.2217/rme-2018-0041
PMID: 30488785
url
https://doi.org/10.2217/rme-2018-0041View
Published (Version of record) Open Access

Abstract

Aim: A virus- and oncogene-free induced pluripotent stem cell (iPSC) reprogramming method was developed with cord blood-derived mononuclear cells (CBDMNC) and peripheral blood mononuclear cells (PBMNC) from patients with genetic lung diseases. Method: iPSC reprogramming used small molecules, hematopoietic stem cell (HSC) expansion media and episomal vectors that lacked Myc and Lin28. Results: All iPSC colonies were fully reprogrammed based on SSEA4 expression. A total of 300,000 CBDMNC was the optimal cell number for cell reprogramming, which was associated with a 13-fold increase in CD34+ cells upon exposure to HSC media. Cell reprogramming was not observed in the absence of HSC expansion media. The method also reprogrammed PBMNC in patients with cystic fibrosis or -1antitrypsin deficiency. Oncogene-free iPSC cell lines differentiated into all three germ cell lineages. Conclusion: This iPSC reprogramming approach satisfies an important regulatory requirement for iPSC-based cell therapies with lower clinical risk from CBDMNC and PBMNC. [GRAPHICS] .
 Cell & Tissue Engineering Cell Biology Engineering Engineering, Biomedical Life Sciences & Biomedicine Science & Technology Technology

Details

Metrics

8 Record Views
6 Times Cited - Web of Science
Logo image