Journal article
Viscoelastic gel formulations enhance airway epithelial gene transfer with viral vectors
American journal of respiratory cell and molecular biology, Vol.32(5), pp.404-410
05/2005
DOI: 10.1165/rcmb.2004-0410OC
PMID: 15695737
Abstract
Advances in gene transfer to the conducting airways for the treatment of pulmonary diseases such as cystic fibrosis have identified several vector classes that transduce airway epithelia in vitro and in animal models. One barrier to epithelial gene transfer is the rapid removal of materials from the airway surface via mucociliary clearance. This host defense mechanism limits gene transfer efficiency to airway epithelial cells. Here we show that formulation of gene transfer vectors with viscoelastic gels provides longer epithelial residence time and increases vector-mediated gene transfer efficiency. Gene transfer with adenoviral, adeno-associated, and lentiviral vectors all significantly improved after formulation with viscoelastic gels designed to slow mucociliary clearance. Importantly, viscoelastic gel formulations enhanced vector transduction to the conducting airways, the desired treatment target for diseases such as cystic fibrosis.
Details
- Title: Subtitle
- Viscoelastic gel formulations enhance airway epithelial gene transfer with viral vectors
- Creators
- Patrick L Sinn - Department of Pediatrics, 240G EMRB, The University of Iowa, Iowa City, IA 52242, USAAnkur J ShahMaureen D DonovanPaul B McCray Jr
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory cell and molecular biology, Vol.32(5), pp.404-410
- DOI
- 10.1165/rcmb.2004-0410OC
- PMID
- 15695737
- ISSN
- 1044-1549
- eISSN
- 1535-4989
- Grant note
- HL 51670 / NHLBI NIH HHS R01 HL 61460 / NHLBI NIH HHS P30 DK 54759 / NIDDK NIH HHS
- Language
- English
- Date published
- 05/2005
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Pharmaceutical Sciences and Experimental Therapeutics; Internal Medicine
- Record Identifier
- 9984093506202771
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