Journal article
Visualizing hypothalamic network dynamics for appetitive and consummatory behaviors
Cell (Cambridge), Vol.160(3), pp.516-527
01/29/2015
DOI: 10.1016/j.cell.2014.12.026
PMCID: PMC4312416
PMID: 25635459
Abstract
Optimally orchestrating complex behavioral states, such as the pursuit and consumption of food, is critical for an organism's survival. The lateral hypothalamus (LH) is a neuroanatomical region essential for appetitive and consummatory behaviors, but whether individual neurons within the LH differentially contribute to these interconnected processes is unknown. Here, we show that selective optogenetic stimulation of a molecularly defined subset of LH GABAergic (Vgat-expressing) neurons enhances both appetitive and consummatory behaviors, whereas genetic ablation of these neurons reduced these phenotypes. Furthermore, this targeted LH subpopulation is distinct from cells containing the feeding-related neuropeptides, melanin-concentrating hormone (MCH), and orexin (Orx). Employing in vivo calcium imaging in freely behaving mice to record activity dynamics from hundreds of cells, we identified individual LH GABAergic neurons that preferentially encode aspects of either appetitive or consummatory behaviors, but rarely both. These tightly regulated, yet highly intertwined, behavioral processes are thus dissociable at the cellular level.
Details
- Title: Subtitle
- Visualizing hypothalamic network dynamics for appetitive and consummatory behaviors
- Creators
- Joshua H Jennings - Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA; Curriculum in Neurobiology, University of North Carolina, Chapel Hill, NC 27599, USA; Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USARandall L Ung - Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA; Curriculum in Neurobiology, University of North Carolina, Chapel Hill, NC 27599, USA; Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USAShanna L Resendez - Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA; Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USAAlice M Stamatakis - Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA; Curriculum in Neurobiology, University of North Carolina, Chapel Hill, NC 27599, USA; Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USAJohnathon G Taylor - Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USAJonathan Huang - Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USAKatie Veleta - Curriculum in Neurobiology, University of North Carolina, Chapel Hill, NC 27599, USAPranish A Kantak - Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USAMegumi Aita - Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USAKelson Shilling-Scrivo - Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USACharu Ramakrishnan - Department of Bioengineering, Stanford University, Stanford, CA 94305, USAKarl Deisseroth - Department of Bioengineering, Stanford University, Stanford, CA 94305, USAStephani Otte - Inscopix Inc., Palo Alto, CA 94303, USAGarret D Stuber - Departments of Psychiatry and Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA; Curriculum in Neurobiology, University of North Carolina, Chapel Hill, NC 27599, USA. Electronic address: gstuber@med.unc.edu
- Resource Type
- Journal article
- Publication Details
- Cell (Cambridge), Vol.160(3), pp.516-527
- Publisher
- United States
- DOI
- 10.1016/j.cell.2014.12.026
- PMID
- 25635459
- PMCID
- PMC4312416
- ISSN
- 0092-8674
- eISSN
- 1097-4172
- Grant note
- DA034472 / NIDA NIH HHS MH104013 / NIMH NIH HHS T32 NS007431 / NINDS NIH HHS DA032750 / NIDA NIH HHS R01 MH099647 / NIMH NIH HHS R01 MH075957 / NIMH NIH HHS F31 DA034472 / NIDA NIH HHS U54 HD079124 / NICHD NIH HHS R37 DA035377 / NIDA NIH HHS T32 GM008719 / NIGMS NIH HHS DA038168 / NIDA NIH HHS R01 DA038168 / NIDA NIH HHS T32HD040127 / NICHD NIH HHS P30 NS045892 / NINDS NIH HHS T32 HD040127 / NICHD NIH HHS R01 DA032750 / NIDA NIH HHS F31 MH104013 / NIMH NIH HHS P60 AA011605 / NIAAA NIH HHS
- Language
- English
- Date published
- 01/29/2015
- Academic Unit
- Periodontics
- Record Identifier
- 9984065815602771
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