Vitamin D-mediated effects on airway innate immunity in vitro

Emma M Stapleton; Kathy Keck; Robert Windisch; Mallory R Stroik; Andrew L Thurman; Joseph Zabner; Ian M Thornell; Alejandro A Pezzulo; Julia Klesney-Tait; Alejandro P Comellas

doi:10.1371/journal.pone.0269647

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Vitamin D-mediated effects on airway innate immunity in vitro

Journal article

Open access

Peer reviewed

Vitamin D-mediated effects on airway innate immunity in vitro

Emma M Stapleton, Kathy Keck, Robert Windisch, Mallory R Stroik, Andrew L Thurman, Joseph Zabner, Ian M Thornell, Alejandro A Pezzulo, Julia Klesney-Tait and Alejandro P Comellas

PloS one, Vol.17(6), pp.e0269647-e0269647

2022

DOI: 10.1371/journal.pone.0269647

PMCID: PMC9170100

PMID: 35666753

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Abstract

Vitamin D supplementation has been suggested to enhance immunity during respiratory infection season. We tested the effect of active vitamin D (calcitriol) supplementation on key airway innate immune mechanisms in vitro. Primary human airway epithelial cells (hAECs) grown at the air liquid interface were supplemented with 10-7 M calcitriol for 24 hours (or a time course) and their antimicrobial airway surface liquid (ASL) was tested for pH, viscoscity, and antibacterial and antiviral properties. We also tested hAEC ciliary beat frequency (CBF). Next, we assessed alterations to hAEC gene expression using RNA sequencing, and based on results, we measured neutrophil migration across hAECs. Calcitriol supplementation enhanced ASL bacterial killing of Staphylococcus aureus (p = 0.02) but did not enhance its antiviral activity against 229E-CoV. It had no effect on ASL pH or viscosity at three timepoints. Lastly, it did not affect hAEC CBF or neutrophil migration, although there was a trend of enhanced migration in the presence of a neutrophil chemokine (p = 0.09). Supplementation significantly altered hAEC gene expression, primarily of AMP-related genes including CAMP and TREM1. While vitamin D supplementation did not have effects on many airway innate immune mechanisms, it may provide a useful tool to resolve respiratory bacterial infections.

Antiviral Agents - metabolism

Calcitriol - metabolism

Cells, Cultured

Epithelial Cells - metabolism

Humans

Immunity, Innate

Vitamin D - metabolism

Vitamin D - pharmacology

Vitamins - metabolism

Details

Title: Subtitle: Vitamin D-mediated effects on airway innate immunity in vitro
Creators: Emma M Stapleton - Roy J. and Lucille A. Carver College of Medicine
Kathy Keck - Roy J. and Lucille A. Carver College of Medicine
Robert Windisch - Roy J. and Lucille A. Carver College of Medicine
Mallory R Stroik - University of Iowa
Andrew L Thurman - Roy J. and Lucille A. Carver College of Medicine
Joseph Zabner - University of Iowa
Ian M Thornell - Roy J. and Lucille A. Carver College of Medicine
Alejandro A Pezzulo - University of Iowa
Julia Klesney-Tait - University of Iowa
Alejandro P Comellas - Roy J. and Lucille A. Carver College of Medicine
Resource Type: Journal article
Publication Details: PloS one, Vol.17(6), pp.e0269647-e0269647
DOI: 10.1371/journal.pone.0269647
PMID: 35666753
PMCID: PMC9170100
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Grant note: DOI: 10.13039/100000050, name: National Heart, Lung, and Blood Institute, award: 5P01HL091842-13
Language: English
Date published: 2022
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; ICTS; Iowa Neuroscience Institute; Internal Medicine
Record Identifier: 9984359834202771

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