Journal article
Voltage-dependent and Ca2(+)-activated ion channels in human neutrophils
The Journal of clinical investigation, Vol.85(2), pp.491-498
02/1990
DOI: 10.1172/JCI114464
PMCID: PMC296450
PMID: 2153708
Abstract
To investigate the regulation of membrane voltage and transmembrane ion fluxes in human neutrophils, we studied plasma membrane currents using the whole-cell patch-clamp method. We observed three distinct ion channel currents: (a) a voltage-dependent K+ current, (b) a Ca2(+)-activated K+ current, and (c) a Ca2(+)-activated Cl- current. The voltage-dependent K+ current was found in cells at rest. Its conductive properties suggested an inwardly rectifying channel. The channel was activated at membrane potentials more positive than -60 mV, suggesting that it may determine the resting membrane potential of neutrophils. Activation of neutrophils by the Ca2+ ionophore ionomycin led to an increase in whole-cell K+ and Cl- currents. The Ca2(+)-activated K+ channel differed from the voltage-dependent K+ channel because it was insensitive to voltage, because it rectified outwardly, and because the voltage-sensitive K+ channel was Ca2(+)-independent. The Ca2(+)-activated Cl- channel showed outward rectification and no apparent voltage dependency. The Ca2(+)-activated K+ and Cl- channels may play a role in cell volume homeostasis and/or cellular activation.
Details
- Title: Subtitle
- Voltage-dependent and Ca2(+)-activated ion channels in human neutrophils
- Creators
- Karl-Heinz Krause - Howard Hughes Medical Institute, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242Michael J Welsh
- Resource Type
- Journal article
- Publication Details
- The Journal of clinical investigation, Vol.85(2), pp.491-498
- Publisher
- American Society for Clinical Investigation
- DOI
- 10.1172/JCI114464
- PMID
- 2153708
- PMCID
- PMC296450
- ISSN
- 0021-9738
- eISSN
- 1558-8238
- Grant note
- R01 AI020866 / NIAID NIH HHS\r\nAI 20866 / NIAID NIH HHS\r\nHL 42385 / NHLBI NIH HHS
- Language
- English
- Date published
- 02/1990
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020756402771
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