Water intake during the development of renal hypertension (2K-1C) in mice

Claudia M Santos; Ralph F Johnson; Terry G Beltz; Robert L Thunhorst; Alan Kim Johnson

doi:10.1016/j.physbeh.2005.05.013

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Water intake during the development of renal hypertension (2K-1C) in mice

Journal article

Peer reviewed

Water intake during the development of renal hypertension (2K-1C) in mice

Claudia M Santos, Ralph F Johnson, Terry G Beltz, Robert L Thunhorst and Alan Kim Johnson

Physiology & behavior, Vol.85(4), pp.512-516

2005

DOI: 10.1016/j.physbeh.2005.05.013

PMID: 15992836

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Abstract

For both practical and methodological reasons, mice have been the most widely employed species for development of transgenic and gene knockin and knockout animals. However, basic behavioral and physiology control and regulatory mechanisms in mice are not well characterized. To broaden our understanding of the processes maintaining body fluid and blood pressure homeostasis in the mouse, the objectives of this study were to evaluate voluntary water, and sodium intakes during the development of renal hypertension and to examine the relationship between hypertension and the quantities of water and salt ingested. In male, C57BL/6J mice, two-kidney, one-clip renal hypertension (2K-1C) was induced, and water and 1.8% NaCl intakes were monitored for 2 weeks. At the end of this period, all animals received arterial catheters for direct recording of blood pressure. The mice that received renal artery clips were sorted into hypertensive (152 ± 4 mm Hg) and normotensive (122 ± 2 mm Hg) groups and were compared to control (117 ± 4 mm Hg) animals that underwent a sham renal clipping procedure. Hypertensive 2K-1C animals had significantly elevated water intake compared to control animals. On most of the postsurgical days, the normotensive 2K-1C animals did not display increased water intake in comparison to the control group. No significant effect was detected for 1.8% saline intake between any of the pairs of groups. In summary, the reduction of blood flow to a single kidney in the 2K-1C model of renal hypertension induces high blood pressure accompanied by sustained hyperdipsia in the mouse.

Biological and medical sciences

Fundamental and applied biological sciences. Psychology

Metabolisms and neurohumoral controls

Vertebrates: anatomy and physiology, studies on body, several organs or systems

Water and mineral metabolism. Osmoregulation. Acidobasic balance

Details

Title: Subtitle: Water intake during the development of renal hypertension (2K-1C) in mice
Creators: Claudia M Santos - Departments of Psychology, Pharmacology, Exercise Science and the Cardiovascular Center, University of Iowa, Iowa City, IA 52242, United States
Ralph F Johnson - Departments of Psychology, Pharmacology, Exercise Science and the Cardiovascular Center, University of Iowa, Iowa City, IA 52242, United States
Terry G Beltz - Departments of Psychology, Pharmacology, Exercise Science and the Cardiovascular Center, University of Iowa, Iowa City, IA 52242, United States
Robert L Thunhorst - Departments of Psychology, Pharmacology, Exercise Science and the Cardiovascular Center, University of Iowa, Iowa City, IA 52242, United States
Alan Kim Johnson - Departments of Psychology, Pharmacology, Exercise Science and the Cardiovascular Center, University of Iowa, Iowa City, IA 52242, United States
Resource Type: Journal article
Publication Details: Physiology & behavior, Vol.85(4), pp.512-516
DOI: 10.1016/j.physbeh.2005.05.013
PMID: 15992836
NLM abbreviation: Physiol Behav
ISSN: 0031-9384
eISSN: 1873-507X
Publisher: Elsevier
Language: English
Date published: 2005
Academic Unit: Psychological and Brain Sciences; Neuroscience and Pharmacology; Health, Sport, and Human Physiology
Record Identifier: 9984213409402771

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