Journal article
West Nile Virus Infection in Human and Mouse Cornea Tissue
The American journal of tropical medicine and hygiene, Vol.95(5), pp.1185-1191
11/02/2016
DOI: 10.4269/ajtmh.16-0256
PMCID: PMC5094237
PMID: 27672204
Abstract
The purpose of this study was to determine the in vitro and ex vivo susceptibility of human corneal cells to West Nile virus (WNV) infection and evaluate the ability of the virus to disseminate to the corneas of infected mice. Human corneal epithelial cells were challenged with WNV, incubated for 1-6 days, and tested for evidence of WNV infection. Viral RNA and antigen were detected at every time point, and the virus reached a peak titer of 2.5 × 10 plaque-forming units (pfu)/mL at 3 days postinoculation (PI). Corneas procured from donors were incubated in culture dishes containing WNV for 1-5 days and tested for evidence of WNV. Viral RNA and antigen were detected, and the virus reached a mean peak titer of 4.9 × 10 pfu/mL at 5 days PI. Mice were inoculated intraperitoneally with WNV, and their eyes were harvested at 2, 5, and 8 days PI and tested for evidence of WNV. Viral RNA was detected in corneas of four of nine systemically infected mice as early as 2 days PI. We conclude that human corneal cells support WNV replication in vitro and ex vivo, and WNV may disseminate into the corneas of experimentally infected mice. These findings indicate that corneal transmission cannot be ruled out as a novel mode of human-to-human WNV transmission and additional experiments should be conducted to assess this risk further.
Details
- Title: Subtitle
- West Nile Virus Infection in Human and Mouse Cornea Tissue
- Creators
- Bradley J Blitvich - Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IowaTian Wang - The University of Texas Medical Branch at GalvestonVandana Saxena - Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TexasShemin Zeng - Stephen A. Wynn Institute for Vision Research, University of Iowa, Iowa City, IowaKaren M Harmon - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IowaMatthew D Raymond - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IowaKenneth M Goins - Iowa Lions Eye Bank, Coralville, IowaCynthia R Reed - Iowa Lions Eye Bank, Coralville, IowaRobert F Mullins - Stephen A. Wynn Institute for Vision Research, University of Iowa, Iowa City, IowaMark A Greiner - Iowa Lions Eye Bank, Coralville, Iowa
- Resource Type
- Journal article
- Publication Details
- The American journal of tropical medicine and hygiene, Vol.95(5), pp.1185-1191
- Publisher
- United States
- DOI
- 10.4269/ajtmh.16-0256
- PMID
- 27672204
- PMCID
- PMC5094237
- ISSN
- 0002-9637
- eISSN
- 1476-1645
- Language
- English
- Date published
- 11/02/2016
- Academic Unit
- Internal Medicine; Ophthalmology and Visual Sciences
- Record Identifier
- 9983980054302771
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