Journal article
White Rot Fungi Produce Novel Tire Wear Compound Metabolites and Reveal Underappreciated Amino Acid Conjugation Pathways
Environmental science & technology letters, Vol.9(5), pp.391-399
05/10/2022
DOI: 10.1021/acs.estlett.2c00114
PMCID: PMC9100321
Appears in UI Libraries Support Open Access
Abstract
There is increasing concern about tire wear compounds (TWCs) in surface water and stormwater as evidence grows on their toxicity and widespread detection in the environment. Because TWCs are prevalent in stormwater, there is a need to understand fate and treatment options including biotransformation in green infrastructure (e.g., bioretention). Particularly, fungal biotransformation is not well-studied in a stormwater context despite the known ability of certain fungi to remove recalcitrant contaminants. Here, we report the first study on fungal biotransformation of the TWCs acetanilide and hexamethoxymethylmelamine (HMMM). We found that the model white rot fungus, Trametes versicolor, removed 81.9% and 69.6% of acetanilide and HMMM, respectively, with no significant sorption to biomass. The bicyclic amine 1,3-diphenylguanidine was not removed. Additionally, we identified novel TWC metabolites using semi-untargeted metabolomics via high-resolution mass spectrometry. Key metabolites include multiple isomers of HMMM biotransformation products, melamine as a possible “dead-end” product of HMMM (verified with an authentic standard), and a glutamine-conjugated product of acetanilide. These metabolites have implications for environmental toxicity and treatment. Our discovery of the first fungal glutamine-conjugated product highlights the need to investigate amino acid conjugation as an important pathway in biotransformation of contaminants, with implications in other fields including natural products discovery.
Details
- Title: Subtitle
- White Rot Fungi Produce Novel Tire Wear Compound Metabolites and Reveal Underappreciated Amino Acid Conjugation Pathways
- Creators
- Erica A WienerGregory H LeFevre - University of Iowa, Civil and Environmental Engineering
- Resource Type
- Journal article
- Publication Details
- Environmental science & technology letters, Vol.9(5), pp.391-399
- DOI
- 10.1021/acs.estlett.2c00114
- PMCID
- PMC9100321
- ISSN
- 2328-8930
- Publisher
- American Chemical Society
- Grants
- Language
- English
- Electronic publication date
- 03/18/2022
- Date published
- 05/10/2022
- Academic Unit
- Civil and Environmental Engineering; IIHR--Hydroscience and Engineering
- Record Identifier
- 9984228055602771
Metrics
93 Record Views