Journal article
Whole-Genome Sequencing and Bioinformatic Analysis of Environmental, Agricultural, and Human Campylobacter jejuni Isolates From East Tennessee
Frontiers in microbiology, Vol.11(na), pp.571064-571064
11/05/2020
DOI: 10.3389/fmicb.2020.571064
PMCID: PMC7674308
PMID: 33224113
Abstract
As a leading cause of bacterial-derived gastroenteritis worldwide, Campylobacter jejuni has a significant impact on human health in both the developed and developing worlds. Despite its prevalence as a human pathogen, the source of these infections remains poorly understood due to the mutation frequency of the organism and past limitations of whole genome analysis. Recent advances in both whole genome sequencing and computational methods have allowed for the high-resolution analysis of intraspecies diversity, leading multiple groups to postulate that these approaches may be used to identify the sources of Campylobacter jejuni infection. To address this hypothesis, our group conducted a regionally and temporally restricted sampling of agricultural and environmental Campylobacter sources and compared isolated C. jejuni genomes to those that caused human infections in the same region during the same time period. Through a network analysis comparing genomes from various sources, we found that human C. jejuni isolates clustered with those isolated from cattle and chickens, indicating these as potential sources of human infection in the region.
Details
- Title: Subtitle
- Whole-Genome Sequencing and Bioinformatic Analysis of Environmental, Agricultural, and Human Campylobacter jejuni Isolates From East Tennessee
- Creators
- Brittni R. Kelley - University of Tennessee at KnoxvilleJ. Christopher Ellis - Oak Ridge National LaboratoryAnnabel Large - Oak Ridge National LaboratoryLiesel G. Schneider - University of Tennessee at KnoxvilleDaniel Jacobson - University of Tennessee at KnoxvilleJeremiah G. Johnson - University of Tennessee at Knoxville
- Resource Type
- Journal article
- Publication Details
- Frontiers in microbiology, Vol.11(na), pp.571064-571064
- Publisher
- Frontiers Media Sa
- DOI
- 10.3389/fmicb.2020.571064
- PMID
- 33224113
- PMCID
- PMC7674308
- ISSN
- 1664-302X
- eISSN
- 1664-302X
- Number of pages
- 17
- Grant note
- Department of Energy; United States Department of Energy (DOE) R013318090 / University of Tennessee Plant Microbe Interface (PMI) Scientific Focus Area by the Office of Biological and Environmental Research in the DOE Office of Science 8321 / Oak Ridge National Laboratory's, Laboratory Directed Research and Development (LDRD) program R013318090 / Oak Ridge National Labs (ORNL) JDRD University of Tennessee DE-AC05-00OR22725 / U.S. Department of Energy; United States Department of Energy (DOE)
- Language
- English
- Date published
- 11/05/2020
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984696707502771
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