Whole genomic analysis of a porcine-like human G5P[6] rotavirus strain isolated from a child with diarrhoea and encephalopathy in Japan

Satoshi Komoto; Yoshimasa Maeno; Mayuko Tomita; Tsuyoshi Matsuoka; Masaharu Ohfu; Toshifumi Yodoshi; Hideki Akeda; Koki Taniguchi

doi:10.1099/vir.0.051011-0

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Whole genomic analysis of a porcine-like human G5P[6] rotavirus strain isolated from a child with diarrhoea and encephalopathy in Japan

Journal article

Peer reviewed

Whole genomic analysis of a porcine-like human G5P[6] rotavirus strain isolated from a child with diarrhoea and encephalopathy in Japan

Satoshi Komoto, Yoshimasa Maeno, Mayuko Tomita, Tsuyoshi Matsuoka, Masaharu Ohfu, Toshifumi Yodoshi, Hideki Akeda and Koki Taniguchi

Journal of general virology, Vol.94(Pt 7), pp.1568-1575

07/01/2013

DOI: 10.1099/vir.0.051011-0

PMID: 23515025

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Abstract

An unusual rotavirus strain, Ryukyu-1120, with G5P[6] genotypes (RVA/Human-wt/JPN/Ryukyu-1120/2011/G5P[6]) was identified in a stool specimen from a hospitalized child aged 4 years who showed diarrhoea and encephalopathy. In this study, we sequenced and characterized the complete genome of strain Ryukyu-1120. On whole genomic analysis, this strain was found to have a unique genotype constellation: G5-P[6]-I5-R1-C1-M1-A8-N1-T1-E1-H1. The VP6 and NSP1 genotypes I5 and A8 are those commonly found in porcine strains. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain Ryukyu-1120 appeared to be of porcine origin. Thus, strain Ryukyu-1120 was found to have a porcine rotavirus genetic backbone and is likely to be of porcine origin. To our knowledge, this is the first report of whole-genome-based characterization of the emerging G5P[6] strains in Asian countries. Our observations will provide important insights into the origin of G5P[6] strains and the dynamic interactions between human and porcine rotavirus strains.

Animals

Antigens, Viral - genetics

Capsid Proteins - genetics

Diarrhea - virology

Encephalitis, Viral - virology

Feces

Genome, Viral - genetics

Genomics - methods

Genotype

Humans

Japan

Molecular Sequence Data

Phylogeny

Polymerase Chain Reaction

Rotavirus - classification

Rotavirus - genetics

Rotavirus - isolation & purification

Rotavirus Infections - virology

Sequence Analysis, DNA

Swine - virology

Viral Nonstructural Proteins - genetics

Viral Proteins - genetics

Details

Title: Subtitle: Whole genomic analysis of a porcine-like human G5P[6] rotavirus strain isolated from a child with diarrhoea and encephalopathy in Japan
Creators: Satoshi Komoto - Fujita Health University
Yoshimasa Maeno - Fujita Health University
Mayuko Tomita - Fujita Health University
Tsuyoshi Matsuoka - Children's Medical Center
Masaharu Ohfu - Children's Medical Center
Toshifumi Yodoshi - Children's Medical Center
Hideki Akeda - Children's Medical Center
Koki Taniguchi - Fujita Health University
Resource Type: Journal article
Publication Details: Journal of general virology, Vol.94(Pt 7), pp.1568-1575
DOI: 10.1099/vir.0.051011-0
PMID: 23515025
ISSN: 0022-1317
eISSN: 1465-2099
Language: English
Date published: 07/01/2013
Academic Unit: Stead Family Department of Pediatrics; Gastroenterology, Hepatology, Pancreatology, and Nutrition
Record Identifier: 9985214106702771

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