Journal article
Whole-mount analysis reveals normal numbers of dopaminergic neurons following misexpression of α-Synuclein in Drosophila
genesis, Vol.41(4), pp.154-159
04/2005
DOI: 10.1002/gene.20106
PMID: 15789427
Abstract
Previously published reports have suggested that misexpression of alpha-Synuclein in the Drosophila central nervous system causes neurodegeneration and progressive age-dependent locomotor dysfunction similar to pathologic and clinical manifestations of Parkinson's disease. The number of dopaminergic (DA) neurons in these studies was assessed using immunohistochemistry with an anti-tyrosine hydroxylase antibody on sequential paraffin sections of fly brains. In contrast, we do not observe any DA cell loss in alpha-Synuclein expressing fly brains when using whole-mount immunohistochemistry as an assay. Our results suggest that the DA cell loss observed with misexpression of alpha-Synuclein is not fully penetrant under a variety of experimental conditions and that this may complicate interpretation of such experiments.
Details
- Title: Subtitle
- Whole-mount analysis reveals normal numbers of dopaminergic neurons following misexpression of α-Synuclein in Drosophila
- Creators
- Yakov Pesah - Division of Neuroscience, Baylor College of Medicine, Houston, TexasHeather Burgess - Department of Pathology, Baylor College of Medicine, Houston, TexasBrooke Middlebrooks - Department of Pathology, Baylor College of Medicine, Houston, TexasKari Ronningen - Department of Pathology, Baylor College of Medicine, Houston, TexasJude Prosser - Department of Molecular Science, AstraZeneca Pharmaceuticals, Wilmington, DelawareVijaya Tirunagaru - Department of Molecular Science, AstraZeneca Pharmaceuticals, Wilmington, DelawareJohn Zysk - Department of Molecular Science, AstraZeneca Pharmaceuticals, Wilmington, DelawareGraeme Mardon - Division of Neuroscience, Baylor College of Medicine, Houston, Texas
- Resource Type
- Journal article
- Publication Details
- genesis, Vol.41(4), pp.154-159
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/gene.20106
- PMID
- 15789427
- ISSN
- 1526-954X
- eISSN
- 1520-6408
- Number of pages
- 6
- Language
- English
- Date published
- 04/2005
- Academic Unit
- Critical Care; Stead Family Department of Pediatrics
- Record Identifier
- 9984093336002771
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