Journal article
Whole transcriptome responses among females of the filariasis and arbovirus vector mosquito Culex pipiens implicate TGF-β signaling and chromatin modification as key drivers of diapause induction
Functional & integrative genomics, Vol.15(4), pp.439-447
07/2015
DOI: 10.1007/s10142-015-0432-5
PMCID: PMC4492872
PMID: 25634120
Abstract
Culex pipiens mosquitoes are important disease vectors inhabiting temperate zones, worldwide. The seasonal reduction in temperature and photoperiod accompanying late summer and early fall prompts female mosquitoes to enter diapause, a stage of developmental arrest and physiological conditioning that enhances survival during the winter months. To investigate the molecular mechanisms underlying diapause induction, we used custom whole transcriptome microarrays to identify differences in gene expression following exposure to nondiapause (long days, 25 °C) and diapause-inducing (short days, 18 °C) environmental conditions. Using a two-way ANOVA, we identified 1130 genes that were differentially expressed. We used the expression of these genes across three time points to construct a gene co-expression network comprising five modules. Genes in modules 1, 2, and 3 were largely up-regulated, while genes in modules 4 and 5 were down-regulated when compared to nondiapause conditions. Pathway enrichment analysis of the network modules revealed some potential regulatory mechanisms driving diapause induction. Module 1 was enriched for genes in the TGF-ß and Wnt signaling pathways; module 2 was enriched for genes involved in insect hormone biosynthesis, specifically, ecdysone synthesis; module 3 was enriched for genes involved in chromatin modification; and module 5 was enriched for genes in the circadian rhythm pathway. Our results suggest that TGF-β signaling and chromatin modification are key drivers for the integration of environmental signals into the diapause induction phase in C. pipiens mosquitoes.
Details
- Title: Subtitle
- Whole transcriptome responses among females of the filariasis and arbovirus vector mosquito Culex pipiens implicate TGF-β signaling and chromatin modification as key drivers of diapause induction
- Creators
- Paul Hickner - Eck Institute for Global Health and Department of Biological Sciences University of Notre Dame 107C Galvin Life Sciences Notre Dame IN 46556 USAAkio Mori - Eck Institute for Global Health and Department of Biological Sciences University of Notre Dame 107C Galvin Life Sciences Notre Dame IN 46556 USAErliang Zeng - Department of Computer Science and Engineering University of Notre Dame Notre Dame IN 46556 USAJohn Tan - Eck Institute for Global Health and Department of Biological Sciences University of Notre Dame 107C Galvin Life Sciences Notre Dame IN 46556 USADavid Severson - Eck Institute for Global Health and Department of Biological Sciences University of Notre Dame 107C Galvin Life Sciences Notre Dame IN 46556 USA
- Resource Type
- Journal article
- Publication Details
- Functional & integrative genomics, Vol.15(4), pp.439-447
- DOI
- 10.1007/s10142-015-0432-5
- PMID
- 25634120
- PMCID
- PMC4492872
- NLM abbreviation
- Funct Integr Genomics
- ISSN
- 1438-793X
- eISSN
- 1438-7948
- Publisher
- Springer Berlin Heidelberg; Berlin/Heidelberg
- Language
- English
- Date published
- 07/2015
- Academic Unit
- Preventive and Community Dentistry; Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Biostatistics; Dental Research
- Record Identifier
- 9984065373802771
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