Widespread targeting of nascent transcripts by RsmA in Pseudomonas aeruginosa

Michael J Gebhardt; Tracy K Kambara; Kathryn M Ramsey; Simon L Dove

doi:10.1073/pnas.1917587117

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Widespread targeting of nascent transcripts by RsmA in Pseudomonas aeruginosa

Journal article

Open access

Peer reviewed

Widespread targeting of nascent transcripts by RsmA in Pseudomonas aeruginosa

Michael J Gebhardt, Tracy K Kambara, Kathryn M Ramsey and Simon L Dove

Proceedings of the National Academy of Sciences - PNAS, Vol.117(19), pp.10520-10529

05/12/2020

DOI: 10.1073/pnas.1917587117

PMCID: PMC7229658

PMID: 32332166

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Abstract

In the opportunistic pathogen , RsmA is an RNA-binding protein that plays critical roles in the control of virulence, interbacterial interactions, and biofilm formation. Although RsmA is thought to exert its regulatory effects by binding full-length transcripts, the extent to which RsmA binds nascent transcripts has not been addressed. Moreover, which transcripts are direct targets of this key posttranscriptional regulator is largely unknown. Using chromatin immunoprecipitation coupled with high-throughput DNA sequencing, with cells grown in the presence and absence of the RNA polymerase inhibitor rifampicin, we identify hundreds of nascent transcripts that RsmA associates with in We also find that the RNA chaperone Hfq targets a subset of those nascent transcripts that RsmA associates with and that the two RNA-binding proteins can exert regulatory effects on common targets. Our findings establish that RsmA associates with many transcripts as they are being synthesized in , identify the transcripts targeted by RsmA, and suggest that RsmA and Hfq may act in a combinatorial fashion on certain transcripts. The binding of posttranscriptional regulators to nascent transcripts may be commonplace in bacteria where distinct regulators can function alone or in concert to achieve control over the translation of transcripts as soon as they emerge from RNA polymerase.

posttranscriptional regulator

Hfq

RNA binding proteins

Details

Title: Subtitle: Widespread targeting of nascent transcripts by RsmA in Pseudomonas aeruginosa
Creators: Michael J Gebhardt - Boston Children's Hospital
Tracy K Kambara - Harvard University
Kathryn M Ramsey - Harvard University
Simon L Dove - Harvard University
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.117(19), pp.10520-10529
DOI: 10.1073/pnas.1917587117
PMID: 32332166
PMCID: PMC7229658
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Grant note: R21 AI125876 / NIAID NIH HHS R01 AI143771 / NIAID NIH HHS
Language: English
Date published: 05/12/2020
Academic Unit: Microbiology and Immunology
Record Identifier: 9984297425202771

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