Wild-type Kaposi's sarcoma-associated herpesvirus isolated from the oropharynx of immune-competent individuals has tropism for cultured oral epithelial cells

Karen M Duus; Vivian Lentchitsky; Timothy Wagenaar; Charles Grose; Jennifer Webster-Cyriaque

doi:10.1128/JVI.78.8.4074-4084.2004

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Wild-type Kaposi's sarcoma-associated herpesvirus isolated from the oropharynx of immune-competent individuals has tropism for cultured oral epithelial cells

Journal article

Open access

Peer reviewed

Wild-type Kaposi's sarcoma-associated herpesvirus isolated from the oropharynx of immune-competent individuals has tropism for cultured oral epithelial cells

Karen M Duus, Vivian Lentchitsky, Timothy Wagenaar, Charles Grose and Jennifer Webster-Cyriaque

Journal of virology, Vol.78(8), pp.4074-4084

04/2004

DOI: 10.1128/JVI.78.8.4074-4084.2004

PMCID: PMC374256

PMID: 15047824

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Abstract

Based on the observation that wild-type Kaposi's sarcoma-associated herpesvirus (KSHV) DNA can be detected in the oral cavity of healthy, immunocompetent individuals, we hypothesized that epithelial cells could be infected in vitro by wild-type (WT) KSHV isolated from immunocompetent individuals. Primary oral epithelial (P-EPI) cells and telomerase-immortalized oral epithelial cells were generated from human gingival tissue and were then infected in vitro with WT KSHV isolated from throat wash samples. Markers of lytic and latent KSHV infection were detected in cultures by 24 h postinfection by immunofluorescence confocal microscopic assays. The infectivity of the WT and BCBL virus was blocked by neutralizing antibodies against KSHV gB. The presence of KSHV DNA in these cells was confirmed by real-time PCR amplification of different regions of the viral genome. The significant in vitro viral replication that had occurred was inhibited by ganciclovir and by neutralizing antibodies against gB. When infected cultures were examined by scanning electron microscopy, thousands of KSHV particles were clearly visible across the surfaces of P-EPI cells. The detection of enveloped particles indicated that the infectious cycle had proceeded through assembly and egress. We thus demonstrated that oral WT KSHV isolated from immunocompetent individuals was able to infect and replicate in vitro in a relevant primary cell type. Furthermore, our results provide compelling evidence for KSHV transmission within infected oral epithelial cells derived from healthy, immunocompetent populations.

Cell Line

Virus Replication - drug effects

Antiviral Agents - pharmacology

Microscopy, Electron, Scanning

Herpesviridae Infections - etiology

Humans

Virulence

Cells, Cultured

DNA, Viral - isolation & purification

Herpesvirus 8, Human - genetics

Organ Specificity

Herpesvirus 8, Human - isolation & purification

Oropharynx - virology

Herpesvirus 8, Human - pathogenicity

Models, Biological

Epithelial Cells - virology

Cell Membrane - virology

Immunocompetence

DNA, Viral - genetics

Herpesvirus 8, Human - physiology

Gingiva - virology

Details

Title: Subtitle: Wild-type Kaposi's sarcoma-associated herpesvirus isolated from the oropharynx of immune-competent individuals has tropism for cultured oral epithelial cells
Creators: Karen M Duus - Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Vivian Lentchitsky
Timothy Wagenaar
Charles Grose
Jennifer Webster-Cyriaque
Resource Type: Journal article
Publication Details: Journal of virology, Vol.78(8), pp.4074-4084
DOI: 10.1128/JVI.78.8.4074-4084.2004
PMID: 15047824
PMCID: PMC374256
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Grant note: K23 DE000460 / NIDCR NIH HHS AI 22795 / NIAID NIH HHS AI 56223 / NIAID NIH HHS K23 DE 00460-01 / NIDCR NIH HHS P30 AI050410 / NIAID NIH HHS 9P30AI50410 / NIAID NIH HHS R21 AI056223 / NIAID NIH HHS R01 DE14444-01 / NIDCR NIH HHS R01 AI022795 / NIAID NIH HHS
Language: English
Date published: 04/2004
Academic Unit: Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
Record Identifier: 9984093601602771

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