Journal article
Wild-type minimum effective concentration distributions and epidemiologic cutoff values for caspofungin and Aspergillus spp. as determined by Clinical and Laboratory Standards Institute broth microdilution methods
Diagnostic microbiology and infectious disease, Vol.67(1), pp.56-60
2010
DOI: 10.1016/j.diagmicrobio.2010.01.001
PMID: 20207095
Abstract
Antifungal susceptibility testing of
Aspergillus spp. against caspofungin has been standardized by the Clinical and Laboratory Standards Institute (CLSI). Recent studies have documented breakthrough infections with
Aspergillus spp. for which the minimum effective concentration (MEC) for caspofungin ranged from 0.25 to 8 μg/mL. We tested a collection of 1590 clinical isolates of
Aspergillus spp. (188
Aspergillus flavus, 1187
Aspergillus fumigatus, 114
Aspergillus niger, 71
Aspergillus terreus, and 30
Aspergillus versicolor) against caspofungin using the CLSI broth microdilution method. An epidemiologic cutoff value (ECV) of ≤0.06 μg/mL encompassed the wild-type (WT) MEC distribution (percentage of MECs) of
A. flavus (99.5%),
A. fumigatus (98.7%),
A. niger (100%), and
A. terreus (97.2%), and an ECV of ≤0.12 μg/mL encompassed the WT distribution of
A. versicolor (96.7%). A total of 20 strains showed MECs that were outside the ECVs: 1
A. flavus (0.12 μg/mL), 16
A. fumigatus (0.12 μg/mL [13], 1 μg/mL [1], 2 μg/mL [2]), 2
A. terreus (0.12 [1] and >8 μg/mL [1]), and 1
A. versicolor (4 μg/mL). The establishment of the WT MEC distributions and ECVs for caspofungin and the major species of
Aspergillus will be useful in resistance surveillance and is an important step toward the development of clinical breakpoints.
Details
- Title: Subtitle
- Wild-type minimum effective concentration distributions and epidemiologic cutoff values for caspofungin and Aspergillus spp. as determined by Clinical and Laboratory Standards Institute broth microdilution methods
- Creators
- Michael A Pfaller - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USALinda Boyken - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USARichard J Hollis - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAJennifer Kroeger - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAShawn A Messer - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAShailesh Tendolkar - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USADaniel J Diekema - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.67(1), pp.56-60
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.diagmicrobio.2010.01.001
- PMID
- 20207095
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Language
- English
- Date published
- 2010
- Academic Unit
- Infectious Diseases; Epidemiology; Pathology; Internal Medicine
- Record Identifier
- 9983986256002771
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