Journal article
Wisconsin microbiome study, a cross-sectional investigation of dietary fibre, microbiome composition and antibiotic-resistant organisms: rationale and methods
BMJ open, Vol.8(3), pp.e019450-e019450
03/01/2018
DOI: 10.1136/bmjopen-2017-019450
PMCID: PMC5875638
PMID: 29588324
Abstract
IntroductionPrevention of multidrug-resistant organism (MDRO) infections, such as those caused by methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, fluoroquinolone-resistant Gram-negative bacteria and Clostridium difficile is crucial. Evidence suggests that dietary fibre increases gut microbial diversity, which may help prevent colonisation and subsequent infection by MDROs. The aim of the Winning the War on Antibiotic Resistance (WARRIOR) project is to examine associations of dietary fibre consumption with the composition of the gut microbiota and gut colonisation by MDROs. The secondary purpose of the study is to create a biorepository of multiple body site specimens for future microbiota research.Methods and analysisThe WARRIOR project collects biological specimens, including nasal, oral and skin swabs and saliva and stool samples, along with extensive data on diet and MDRO risk factors, as an ancillary study of the Survey of the Health of Wisconsin (SHOW). The SHOW is a population-based health survey collecting data on several different health determinants and outcomes, as well as objective body measurements and biological specimens. WARRIOR participants include 600 randomly selected Wisconsin residents age 18 and over. Specimens are screened for MDRO colonisation and DNA is extracted for 16S ribosomal RNA-based microbiota sequencing. Data will be analysed to assess the relationship between dietary fibre, the gut microbiota composition and gut MDRO colonisation.Ethics and disseminationThe WARRIOR project is approved by the University of Wisconsin Institutional Review Board. The main results of this study will be published in a peer-reviewed scientific journal.
Details
- Title: Subtitle
- Wisconsin microbiome study, a cross-sectional investigation of dietary fibre, microbiome composition and antibiotic-resistant organisms: rationale and methods
- Creators
- Shoshannah Eggers - University of Wisconsin–MadisonKristen MC Malecki - University of Wisconsin–MadisonPaul Peppard - University of Wisconsin–MadisonJulie Mares - University of Wisconsin–MadisonDaniel Shirley - University of Wisconsin–MadisonSanjay K Shukla - Marshfield ClinicKeith Poulsen - University of Wisconsin–MadisonRonald Gangnon - University of Wisconsin–MadisonMegan Duster - University of Wisconsin–MadisonAshley Kates - University of Wisconsin–MadisonGarret Suen - University of Wisconsin–MadisonAjay K Sethi - University of Wisconsin–MadisonNasia Safdar - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- BMJ open, Vol.8(3), pp.e019450-e019450
- DOI
- 10.1136/bmjopen-2017-019450
- PMID
- 29588324
- PMCID
- PMC5875638
- NLM abbreviation
- BMJ Open
- ISSN
- 2044-6055
- eISSN
- 2044-6055
- Grant note
- name: Wisconsin Partnership Program, School of Medicine and Public Health, University of Wisconsin-Madison
- Language
- English
- Date published
- 03/01/2018
- Academic Unit
- Epidemiology; Injury Prevention Research Center
- Record Identifier
- 9984446412802771
Metrics
4 Record Views