Wnt-5/pipetail functions in vertebrate axis formation as a negative regulator of Wnt/beta-catenin activity

Trudi A Westfall; Ryan Brimeyer; Jen Twedt; Jean Gladon; Andrea Olberding; Makoto Furutani-Seiki; Diane C Slusarski

doi:10.1083/jcb.200303107

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Wnt-5/pipetail functions in vertebrate axis formation as a negative regulator of Wnt/beta-catenin activity

Journal article

Open access

Peer reviewed

Wnt-5/pipetail functions in vertebrate axis formation as a negative regulator of Wnt/beta-catenin activity

Trudi A Westfall, Ryan Brimeyer, Jen Twedt, Jean Gladon, Andrea Olberding, Makoto Furutani-Seiki and Diane C Slusarski

The Journal of cell biology, Vol.162(5), pp.889-898

09/01/2003

DOI: 10.1083/jcb.200303107

PMCID: PMC2172822

PMID: 12952939

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Abstract

We provide genetic evidence defining a role for noncanonical Wnt function in vertebrate axis formation. In zebrafish, misexpression of Wnt-4, -5, and -11 stimulates calcium (Ca2+) release, defining the Wnt/Ca2+ class. We describe genetic interaction between two Wnt/Ca2+ members, Wnt-5 (pipetail) and Wnt-11 (silberblick), and a reduction of Ca2+ release in Wnt-5/pipetail. Embryos genetically depleted of both maternal and zygotic Wnt-5 product exhibit cell movement defects as well as hyperdorsalization and axis-duplication phenotypes. The dorsalized phenotypes result from increased beta-catenin accumulation and activation of downstream genes. The Wnt-5 loss-of-function defect is consistent with Ca2+ modulation having an antagonistic interaction with Wnt/beta-catenin signaling.

Morphogenesis

Phenotype

Zebrafish - anatomy & histology

Calcium - metabolism

Homeodomain Proteins - metabolism

Glycoproteins - metabolism

Wnt Proteins

Wnt-5a Protein

Zebrafish - embryology

In Situ Hybridization

Body Patterning

Cytoskeletal Proteins - metabolism

Female

Calcium-Calmodulin-Dependent Protein Kinase Type 2

Proto-Oncogene Proteins - metabolism

beta Catenin

Zebrafish Proteins - metabolism

Mitogens - metabolism

Proto-Oncogene Proteins - genetics

Homeodomain Proteins - genetics

Animals

Zebrafish - physiology

Signal Transduction - physiology

Trans-Activators - metabolism

Zebrafish Proteins - genetics

Calcium-Calmodulin-Dependent Protein Kinases - metabolism

Details

Title: Subtitle: Wnt-5/pipetail functions in vertebrate axis formation as a negative regulator of Wnt/beta-catenin activity
Creators: Trudi A Westfall - University of Iowa, Biology
Ryan Brimeyer
Jen Twedt
Jean Gladon
Andrea Olberding
Makoto Furutani-Seiki
Diane C Slusarski
Resource Type: Journal article
Publication Details: The Journal of cell biology, Vol.162(5), pp.889-898
Publisher: United States
DOI: 10.1083/jcb.200303107
PMID: 12952939
PMCID: PMC2172822
ISSN: 0021-9525
eISSN: 1540-8140
Language: English
Date published: 09/01/2003
Academic Unit: Biology; Internal Medicine
Record Identifier: 9983991998702771

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